Strain Data Sheet

RBRC05472

Strain Information

Image
BRC No.RBRC05472
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC.Cg-Gt(ROSA)26Sor<tm6.1(CAG-tdTomato)Maoh>
Former Common nameBALB-ROSA26/CAG-tdTomato
H-2 Haplotype
ES Cell lineE14TG2a [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDevelop by Masato Ohtsuka, Tokai University School of Medicine in 2010. The mice were backcrossed to BALB/cAJcl. Extra sequence in the RMCE<ex> was removed by FLP-mediated recombination.
Strain descriptionThe mice having a CAG-tdTomato-polyA cassette at the ROSA26 locus that were generated by RMCE (Recombinase-mediated cassette exchange). The mutant mice exhibit ubiquitous and very bright fluorescence.
Colony maintenanceHomozygote x Homozygote [or Crossing to BALB/cAJcl]
References
Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression.
Ohtsuka M, Ogiwara S, Miura H, Mizutani A, Warita T, Sato M, Imai K, Hozumi K, Sato T, Tanaka M, Kimura M, Inoko H
Nucleic Acids Res., 38, e198 (2010). 20880997

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site6Frt
Gt(ROSA)26Sor
MGI:104735		gene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm6.1(CAG-tdTomato)Maoh>
MGI:5056526		targeted mutation 6.1, Masato Ohtsuka
SA Splice acceptor6SA
lox2272 phage P1 loxP variant6lox2272
tdTomato tandem dimeric Red Fluorescent Protein (Discosoma sp.)6tdTomato CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
SV40 polyA6

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAadenovirus major late transcript splice acceptor sequence (SA), CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Discosoma sp. tdTomato cDNA, SV40 polyA, yeast FRT (flippase recombination target) site, Phage P1 lox2272 site
    Research applicationCell Biology Research
    Cre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nucleic Acids Res., 38, e198 (2010). For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
    DepositorMasato　Ohtsuka (Tokai University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. CAGGS MTA (English)
    4. UCSD MTA
    5. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data