Strain Data Sheet

RBRC05473

Strain Information

Image
BRC No.RBRC05473
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S6-Mad2l2<tm1Myo>
Former Common nameRev7 KO, B6-Mad2l2 KO
H-2 Haplotype
ES Cell lineCSL3[129S6/SvEvTac]
Background strain
Appearance
Strain developmentDeveloped by Yoshiki Murakumo, Nagoya University Graduate School of Medicine in 2009. The mutant mice were backcrossed to C57BL/6.
Strain descriptionRev7 (Mad2l2) knockout mice. Homozygous mutant mice lack germ cells due to apoptosis of primordial germ cell in the middle phase of the embryonic development.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJmsSlc]
References
A human REV7 homolog that interacts with the polymerase zeta catalytic subunit hREV3 and the spindle assembly checkpoint protein hMAD2.
Murakumo Y, Roth T, Ishii H, Rasio D, Numata S, Croce C M, Fishel R
J. Biol. Chem., 275, 4391-4397 (2000). 10660610

The REV7 subunit of DNA polymerase zeta is essential for primordial germ cell maintenance in the mouse.
Watanabe N, Mii S, Asai N, Asai M, Niimi K, Ushida K, Kato T, Enomoto A, Ishii H, Takahashi M, Murakumo Y
J. Biol. Chem., 288(15):10459-10471 (2013). 23463509

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Mad2l2
MGI:1919140		MAD2 mitotic arrest deficient-like 24Mad2l2<tm1Ymu>
MGI:5505390		targeted mutation 1, Yoshiki Murakumo
  • Fanconi anemia complementation group V(MedGEN)
    • neo neomycin resistance gene (E. coli)4 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal double-strand DNA break repair(MP:0010948)

  • abnormal oogenesis(MP:0001931)

  • abnormal spermatogenesis(MP:0001156)

  • absent ovarian follicles(MP:0002777)

  • absent primordial germ cells(MP:0008393)
    • more 18 phenotypes
  • azoospermia(MP:0005159)

  • decreased birth body size(MP:0009703)

  • decreased body size(MP:0001265)

  • decreased body weight(MP:0001262)

  • decreased embryo size(MP:0001698)

  • decreased primordial germ cell number(MP:0008392)

  • decreased testis weight(MP:0004852)

  • female infertility(MP:0001926)

  • increased forebrain apoptosis(MP:0012504)

  • increased primordial germ cell apoptosis(MP:0030935)

  • lethality during fetal growth through weaning, incomplete penetrance(MP:0011112)

  • male infertility(MP:0001925)

  • nervous system phenotype(MP:0003631)

  • ovary atrophy(MP:0004833)

  • seminiferous tubule degeneration(MP:0001154)

  • small ovary(MP:0001127)

  • small testis(MP:0001147)

  • weakness(MP:0000746)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMouse phosphoglycerate kinase promoter, E. coli Neomycin resistance gene, SV40 PolyA signal, Mouse Rev7 gene
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 288(15):10459-10471 (2013). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For profit use of the BIOLOGICAL RESSOURCE, the RECIPIENT must contact with the Intellectual Property Office, Nagoya University to obtain a prior written consent.
    The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorYoshiki Murakumo (Nagoya University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data