Strain Information | |
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Image | |
BRC No. | RBRC05494 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;129P2-Tnfaip3<tm1Homy> |
Former Common name | A20 cKO, B6-Tnfaip3 floxed |
H-2 Haplotype | |
ES Cell line | E14 [129P2/OlaHsd] |
Background strain | |
Appearance | |
Strain development | Developed by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University. E14 ES cells (129P2/OlaHsd) were used. The mutant mice were crossed to C57BL/6N mice. |
Strain description | A20 (Tnfaip3) gene floxed mice. Exon 3 of A20 gene was flanked by loxP sites. Conditional A20 deficient mice can be generated by crossing with tissue-specific Cre mice. |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6NCrlCrlj] |
References | PLoS One, 9(1):e87425 (2014). 24498102 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site10Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP10loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP10loxP |
Ordering Information | |
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Donor DNA | Phage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Tnfaip3 genomic DNA |
Research application | Cre/loxP system FLP/frt system |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. |
Depositor | Hiroaki Honda (Tokyo Women's Medical University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month May 2014 |
BRC mice in Publications |
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Yusuke Yokoyama, Tomohiro Tamachi, Arifumi Iwata, Yuko Maezawa, Kazuyuki Meguro, Masaya Yokota, Hiroaki Takatori, Akira Suto, Kotaro Suzuki, Koichi Hirose, Noritaka Yamaguchi, Hiroaki Honda, Hiroshi Nakajima A20 (Tnfaip3) expressed in CD4+ T cells suppresses Th2 cell-mediated allergic airway inflammation in mice Biochemical and Biophysical Research Communications 629 47-53(2022) 36099784 Liu SS, Lv XX, Liu C, Qi J, Li YX, Wei XP, Li K, Hua F, Cui B, Zhang XW, Yu JJ, Yu JM, Wang F, Shang S, Zhao CX, Hou XY, Yao ZG, Li PP, Li X, Huang B, Hu ZW. Targeting Degradation of the Transcription Factor C/EBPβ Reduces Lung Fibrosis by Restoring Activity of the Ubiquitin-Editing Enzyme A20 in Macrophages. Immunity 51(3) 522-534.e7(2019) 31471107 Montarolo F, Perga S, Tessarolo C, Spadaro M, Martire S, Bertolotto A. TNFAIP3 Deficiency Affects Monocytes, Monocytes-Derived Cells and Microglia in Mice. Int J Mol Sci 21(8) (2020) 32325694 |