Strain Data Sheet

RBRC05501

Strain Information

Image
BRC No.RBRC05501
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain name129-Tmem119<tm1Tfur>
Former Common name129-Obif KO, 129-Obif<tm1Tfur>
H-2 Haplotype
ES Cell lineTC1 [129S6/SvEvTac]
Background strain
Appearance
Strain developmentDeveloped by Takahisa Furukawa, Osaka Bioscience Institute in 2006. 129 background.
Strain descriptionObif, osteoblast induction factor gene knockout mice. Exon 2 of Obif gene was replaced with PGK-neo cassette. Homozygous mutant mice show lower bone mass than controls at 14 days and 8 weeks old.
Colony maintenanceHomozygote x Heterozygote [or Crossing to 129S6/SvEvTac]
References
Isolation and characterization of a novel plasma membrane protein, osteoblast induction factor (obif), associated with osteoblast differentiation.
Kanamoto T, Mizuhashi K, Terada K, Minami T, Yoshikawa H, Furukawa T
BMC Dev. Biol., 9, 70 (2009). 20025746

OBIF, an osteoblast induction factor, plays an essential role in bone formation in association with osteoblastogenesis.
Mizuhashi K, Kanamoto T, Ito M, Moriishi T, Muranishi Y, Omori Y, Terada K, Komori T, Furukawa T
Dev. Growth Differ., 54(4):474-480 (2012). 22416756

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Tmem119
MGI:2385228		transmembrane protein 1195Tmem119 <tm1Tfur>
MGI:5544075		targeted mutation 1, Takahisa Furukawa
neo neomycin resistance gene (E. coli)5 mouse phosphoglycerate kinase promoter (PGK promoter)
SV40 polyA signal5

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal bone trabecula morphology(MP:0010867)

  • decreased body length(MP:0001258)

  • decreased body weight(MP:0001262)

  • decreased bone trabecula number(MP:0010869)

  • decreased compact bone thickness(MP:0000135)
    • more 6 phenotypes
  • decreased trabecular bone connectivity density(MP:0010933)

  • decreased trabecular bone volume(MP:0010879)

  • delayed endochondral bone ossification(MP:0003419)

  • impaired osteoblast differentiation(MP:0011711)

  • postnatal growth retardation(MP:0001732)

  • skeleton phenotype(MP:0005390)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, SV40 Poly A signal, mouse Tmem119 genomic DNA
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Growth Differ., 54(4):474-480 (2012).Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University).
    DepositorTakahisa Furukawa (Osaka Bioscience Institute)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data