Strain Data Sheet

RBRC05512

Strain Information

Image
BRC No.RBRC05512
TypeInbred
SpeciesMus musculus
Strain nameC57BL/10ScCr
Former Common nameB10ScCr
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentMaintained by Gris Galanos, Max-Planck-Institute for Immunobiology
Strain descriptionThis strain is homozygous for the defective lipopolysaccharide and IL-12 interferon responses (Tlr4<lps-del> and Il12rb2<Ifnm-d>).
Colony maintenanceTlr4 : Homozygote x Homozygote ; Il12rb2 : Homozygote x Homozygote Homozygous mutant mice are viable and fertile.
References
Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations in Tlr4 gene.
Poltorak A, He X, Smirnova I, Liu M Y, Van Huffel C, Du X, Birdwell D, Alejos E, Silva M, Galanos C, Freudenberg M, Ricciardi-Castagnoli P, Layton B, Beutler B
Science, 282, 2085-2088 (1998). 9851930

A point mutation in the IL-12R beta 2 gene underlies the IL-12 unresponsiveness of Lps-defective C57BL/10ScCr mice.
Poltorak A, Merlin T, Nielsen P J, Sandra O, Smirnova I, Schupp I, Boehm T, Galanos C, Freudenberg M A
J. Immunol., 167, 2106-2111 (2001). 11489994

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Tlr4
MGI:96824		toll-like receptor 44Tlr4<lps-del>
MGI:1860755		toll-like receptor 4; defective lipopolysaccharide
Il12rb2
MGI:1270861		interleukin 12 receptor, beta 26Il12rb2<Ifnm-d>
MGI:2156401		interferon response to microorgansisms, defective

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal NK cell physiology(MP:0010766)

  • abnormal circulating enzyme level(MP:0001570)

  • abnormal cytokine secretion(MP:0003009)

  • abnormal dendritic cell physiology(MP:0002376)

  • abnormal glial cell physiology(MP:0003690)
    • more 29 phenotypes
  • abnormal granulocyte physiology(MP:0002462)

  • abnormal immune cell physiology(MP:0001819)

  • abnormal macrophage physiology(MP:0002451)

  • abnormal neuron differentiation(MP:0009937)

  • abnormal pulmonary alveolar duct morphology(MP:0002271)

  • abnormal pulmonary alveolus morphology(MP:0002270)

  • abnormal retina layer morphology(MP:0003727)

  • abnormal retina pigment epithelium morphology(MP:0005201)

  • abnormal retina progenitor cell morphology(MP:0006071)

  • abnormal retina rod bipolar cell morphology(MP:0006074)

  • abnormal retina rod cell morphology(MP:0001005)

  • abnormal tumor necrosis factor level(MP:0008752)

  • brain ischemia(MP:0006080)

  • decreased circulating alanine transaminase level(MP:0002942)

  • decreased interferon-gamma secretion(MP:0008567)

  • decreased interleukin-10 secretion(MP:0008661)

  • decreased interleukin-6 secretion(MP:0008706)

  • decreased percent body fat/body weight(MP:0005459)

  • decreased response of heart to induced stress(MP:0004486)

  • decreased susceptibility to injury(MP:0005166)

  • enlarged lung(MP:0004882)

  • hyporesponsive to tactile stimuli(MP:0005498)

  • increased bone mass(MP:0005605)

  • increased bone mineral content(MP:0010123)

  • increased interleukin-4 secretion(MP:0008699)

  • increased spleen weight(MP:0004952)

  • increased susceptibility to bacterial infection(MP:0002412)

  • increased thermal nociceptive threshold(MP:0001973)

  • rectal hemorrhage(MP:0003293)
    • Detailed phenotype data

    Ordering Information

    Donor DNA
    Research applicationImmunology and Inflammation Research
    Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
    DepositorHiroaki Takimoto (Kitasato University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data