Strain Data Sheet

RBRC05517

Strain Information

Image
BRC No.RBRC05517
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-Xbp1<tm1Tiw>
Former Common nameXBP1 KO, B6-Xbp1 KO
H-2 Haplotype
ES Cell lineRF8 [129S4/SvJae]
Background strain
Appearance
Strain developmentDeveloped by Takao Iwawaki, Advanced Science Institute, RIKEN in 2005. RF8 (129S4 derived) ES cells were used. The mutant mice were backcrossed to C57BL/6J for at least 5 times.
Strain descriptionXbp1 gene knockout mice. Exons 2-4 were replaced with a floxed neo cassette. Homozygous mutant mice begin to die around E12.5.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
Function of IRE1 alpha in the placenta is essential for placental development and embryonic viability.
Iwawaki T, Akai R, Yamanaka S, Kohno K
Proc. Natl. Acad. Sci. USA, 106, 16657-16662 (2009). 19805353

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Xbp1
MGI:98970
X-box binding protein 111Xbp1<tm1Tiw>
MGI:4365400
targeted mutation 1, Takao Iwawaki
  • major affective disorder 7(MedGEN)
  • loxP phage P1 loxP11loxP
    loxP phage P1 loxP11loxP
    neo neomycin resistance gene (E. coli)11 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal extraembryonic tissue physiology(MP:0004264)

  • decreased embryo size(MP:0001698)
  • Detailed phenotype data

    Ordering Information

    Donor DNAP1 Phage loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E.coli neo, mouse Xbp1 genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 106, 16657-16662 (2009). The RECIPIENT shall use the BIOLOGICAL RESOURCE in collaboration with the DEPOSITOR (Dr. Takao Iwawaki).
    DepositorTakao Iwawaki (Gunma University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data