Strain Data Sheet

RBRC05522

Strain Information

Image
BRC No.RBRC05522
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S7-Sox9<tm1(cre/ERT2)Haak>
Former Common nameSox9-CreERT2 KI, Sox9-CreERT2 knock-in, B6-Sox9 KI/CreER
H-2 Haplotype
ES Cell lineAB1 [129S7/SvEvBrd-Hprt<+>]
Background strain
Appearance
Strain developmentDeveloped by Haruhiko Akiyama, The University of Texas MD Anderson Cancer Center. The knock-in vector was electroporated into AB1 ES cells. The mutant mice were crossed to C57BL/6.
Strain descriptionSox9-CreER knock-in mice. These mice have a tamoxifen-inducible cre (CreERT2) at the Sox9 locus. IRES-CreERT2-pA and FRT-PGK-neo-bpA cassettes were inserted into the 3’ UTR of exon 3 of the Sox9 locus.
Colony maintenanceHeterozygote x Wild-type [C57BL/6]
References
Sox9-expressing precursors are the cellular origin of the cruciate ligament of the knee joint and the limb tendons.
Soeda T, Deng J M, de Crombrugghe B, Behringer R R, Nakamura T, Akiyama H
Genesis., 48, 635-644 (2010). 20806356

Health Report

Examination Date / Room / Rack2025/01/20Room:3-CRack:GSentinel mouse program
2024/10/21Room:3-CRack:GSentinel mouse program
2024/07/22Room:3-CRack:GSentinel mouse program
2024/04/22Room:3-CRack:GSentinel mouse program
2024/01/22Room:3-CRack:GSentinel mouse program
2023/10/23Room:3-CRack:GSentinel mouse program
2023/07/24Room:3-CRack:GSentinel mouse program
2023/04/24Room:3-CRack:GSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
ERT2 estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)11ERT2
Frt yeast FRT (flippase recombination target) site11Frt
Frt yeast FRT (flippase recombination target) site11Frt
GH Growth hormone polyA (Bovine)11GH
IRES internal ribosomal entry site (EMCV)11
Sox9
MGI:98371		SRY (sex determining region Y)-box 911Sox9<tm1(cre/ERT2)Haak>
MGI:4867441		targeted mutation 1, Haruhiko Akiyama
  • campomelic dysplasia(DisGeNET, MedGEN)
    • cre Phage P1 Cre recombinase11cre
    neo neomycin resistance gene (E. coli)11 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Sox9 genomic DNA, EMCV internal ribosomal entry site (ires), bacteriophage P1 cre recombinase gene, human estrogen receptor binding domain (ERT2), mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, bovine polyA signal, yeast FRT (flipase recombination target) sites
    Research applicationCre/loxP system
    Developmental Biology Research
    FLP/frt system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis., 48, 635-644 (2010). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Haruhiko Akiyama, Kyoto University, is requested. Depositor retains ownership of the Biological Resource, including any Biological Resource contained in any modification. Any Biological Resource delivered pursuant to the Agreement is understood to be experimental in nature and may have hazardous properties. The DEPOSITOR MAKES NO REPRESENTATIONS AND EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED. THERE ARE NO EXPRESS OR IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR THAT THE USE OF THE BIOLOGICAL RESOURCE WILL NOT INFRINGE ANY PATENT, COPYRIGHT, TRADEMARK, OR OTHER PROPRIETARY RIGHTS. In no event shall Depositor be liable for any use by recipient of the Biological Resource for any loss, claim, damage or liability, of whatsoever kind of nature, which may arise from or in connection with the use, handling or storage of the Biological Resource. Except where limited by Federal law, or by the constitution and laws of the state governing the recipient, the recipient agrees to hold harmless Depositor and The University of Texas System and their Regents, officers, agents and employees, from any liability, loss or damage they may suffer as a result of claims, demands, costs or judgments against them arising out of recipient’s use of the Material and the use by recipient of the results obtained from the Research. The Biological Resource and modifications containing the Biological Resource may only be transferred to non-profit institutions for academic research purposes.
    DepositorBenoit de Crombrugghe (The University of Texas M. D. Anderson Cancer Center)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Cre-ERT(2): NOTICE TO RECIPIENT
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Huang S, Gao D, Li Z, He H, Yu X, You X, Wu D, Du Z, Zeng J, Shi X, Hu Q, Nie Y, Zhang Z, Luo Z, Wang D, Zhao Z, Li L, Wang G, Wang L, Zhou Z, Chen D, Yang F.
    Neuronal guidance factor Sema3A inhibits neurite ingrowth and prevents chondrocyte hypertrophy in the degeneration of knee cartilage in mice, monkeys and humans.
    Bone Res 13(1) 4(2025) 39746903
    Ievlev V, Jensen-Cody CC, Lynch TJ, Pai AC, Park S, Shahin W, Wang K, Parekh KR, Engelhardt JF.
    Sox9 and Lef1 Regulate the Fate and Behavior of Airway Glandular Progenitors in Response to Injury.
    Stem Cells 40(8) 778-790(2022) 35639980