Strain Data Sheet

RBRC05523

Strain Information

Image
BRC No.RBRC05523
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S7-Sox9<tm1(cre)Crm>
Former Common nameSox9-Cre KI, Sox9-Cre knock-in, B6-Sox9 KI/Cre
H-2 Haplotype
ES Cell lineAB1 [129S7/SvEvBrd-Hprt<+>]
Background strain
Appearance
Strain developmentDeveloped by Haruhiko Akiyama, The University of Texas MD Anderson Cancer Center. The knock-in vector was electroporated into AB1 ES cells. The mutant mice were crossed to C57BL/6.
Strain descriptionSox9-Cre knock-in mice. These mice have a cre recombinase gene at the Sox9 locus. An IRES-Cre-pA/frt-flanked PGK-neo-bpA cassette was inserted into the 3’ UTR of exon 3 of the Sox9 locus.
Colony maintenanceHeterozygote x Wild-type [C57BL/6]
References
Osteo-chondroprogenitor cells are derived from Sox9 expressing precursors.
Akiyama H, Kim J E, Nakashima K, Balmes G, Iwai N, Deng J M, Zhang Z, Martin J F, Behringer R R, Nakamura T, de Crombrugghe B
Proc. Natl. Acad. Sci. USA, 102, 14665-14670 (2005). 16203988

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site11Frt
Frt yeast FRT (flippase recombination target) site11Frt
IRES internal ribosomal entry site (EMCV)11
Sox9
MGI:98371		SRY (sex determining region Y)-box 911Sox9<tm1(cre)Crm>
MGI:3608931		targeted mutation 3, Benoit de Crombrugghe
  • campomelic dysplasia(DisGeNET, MedGEN)
    • cre Phage P1 Cre recombinase11cre
    neo neomycin resistance gene (E. coli)11 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAEncephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), bacteriophage P1 cre recombinase gene, poly A, yeast FRT (flipase recombination target) sites, mouse PGK promoter, E. coli Neomycin resistance gene, bovine polyA signal, mouse Sox9 genomic DNA
    Research applicationCre/loxP system
    Developmental Biology Research
    FLP/frt system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. U S A, 102, 14665-14670 (2005). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Haruhiko Akiyama, Kyoto University, is requested. Depositor retains ownership of the Biological Resource, including any Biological Resource contained in any modification. Any Biological Resource delivered pursuant to the Agreement is understood to be experimental in nature and may have hazardous properties. The DEPOSITOR MAKES NO REPRESENTATIONS AND EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED. THERE ARE NO EXPRESS OR IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR THAT THE USE OF THE BIOLOGICAL RESOURCE WILL NOT INFRINGE ANY PATENT, COPYRIGHT, TRADEMARK, OR OTHER PROPRIETARY RIGHTS. In no event shall Depositor be liable for any use by recipient of the Biological Resource for any loss, claim, damage or liability, of whatsoever kind of nature, which may arise from or in connection with the use, handling or storage of the Biological Resource. Except where limited by Federal law, or by the constitution and laws of the state governing the recipient, the recipient agrees to hold harmless Depositor and The University of Texas System and their Regents, officers, agents and employees, from any liability, loss or damage they may suffer as a result of claims, demands, costs or judgments against them arising out of recipient’s use of the Material and the use by recipient of the results obtained from the Research. The Biological Resource and modifications containing the Biological Resource may only be transferred to non-profit institutions for academic research purposes.
    DepositorBenoit de Crombrugghe (The University of Texas M. D. Anderson Cancer Center)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data