Strain Data Sheet

RBRC05540

Strain Information

Image
BRC No.RBRC05540
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Brd1<tm1Hko>
Former Common nameBrd1 KO, B6-Brd1 KO
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Atsushi Iwama, Graduate School of Medicine, Chiba University and Akihiko Koseki, RIKEN Research Center for Allergy and Immunology in 2005. The mutant mice were backcrossed to C57BL/6N.
Strain descriptionBrd1 gene knockout mice. Exon 2 of Brd1 gene was replaced with a floxed neo cassette. Homozygous mutant mice show abnormal embryonic development including erythropoiesis (anemia), growth retardation, open neural tube and abnormal lens morphology, and die between E12.5 and E14.5.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrSlc]
References
The Hbo1-Brd1/Brpf2 complex is responsible for global acetylation of H3K14 and required for fetal liver erythropoiesis.
Mishima Y, Miyagi S, Saraya A, Negishi M, Endoh M, Endo T A, Toyoda T, Shinga J, Katsumoto T, Chiba T, Yamaguchi N, Kitabayashi I, Koseki H, Iwama A
Blood, 118, 2443-2453 (2011). 21753189

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Brd1
MGI:1924161		bromodomain containing 115Brd1<tm1Hko>
MGI:5296490		targeted mutation 1, Haruhiko Koseki
loxP phage P1 loxP15loxP
loxP phage P1 loxP15loxP
neo neomycin resistance gene (E. coli)15 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal embryo development(MP:0001672)

  • abnormal embryonic erythropoiesis(MP:0004229)

  • abnormal embryonic hematopoiesis(MP:0003396)

  • abnormal erythropoiesis(MP:0000245)

  • abnormal fetal liver hematopoietic progenitor cell morphology(MP:0011246)
    • more 15 phenotypes
  • abnormal lens morphology(MP:0001303)

  • abnormal liver development(MP:0000596)

  • abnormal optic cup morphology(MP:0004269)

  • anemia(MP:0001577)

  • decreased erythroblast number(MP:0011179)

  • decreased erythroid progenitor cell number(MP:0008973)

  • decreased hepatoblast number(MP:0012243)

  • embryonic growth retardation(MP:0003984)

  • embryonic lethality during organogenesis, incomplete penetrance(MP:0011108)

  • impaired hematopoiesis(MP:0001606)

  • increased apoptosis(MP:0006042)

  • lethality throughout fetal growth and development, complete penetrance(MP:0011099)

  • open neural tube(MP:0000929)

  • pallor(MP:0003717)

  • small liver(MP:0000601)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli Neomycin resistance gene, Mouse Phosphoglycerate kinase promoter (PGK promoter), SV40 PolyA signal, Bacteriophage loxP
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Blood, 118, 2443-2453 (2011).
    DepositorAtsushi Iwama (Chiba University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data