Strain Data Sheet

RBRC05541

Strain Information

Image
BRC No.RBRC05541
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Zfp277<tm1>
Former Common nameZfp277 KO, B6-Zfp277 KO
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Atsushi Iwama, Graduate School of Medicine, Chiba University in 2005. The mutant mice were backcrossed to C57BL/6N.
Strain descriptionZfp277 gene knockout mice. Exon 5 of Zfp277 gene was replaced with a floxed neo cassette. Homozygous mutant mice were born according to Menderian’s law, but the growth retardation and death of homozygous mice were sporadically observed.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrSlc]
References
A novel zinc finger protein Zfp277 mediates transcriptional repression of the Ink4a/arf locus through polycomb repressive complex 1.
Negishi M, Saraya A, Mochizuki S, Helin K, Koseki H, Iwama A
PLoS One, 5, e12373 (2010). 20808772

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Zfp277
MGI:1890393		zinc finger protein 27712Zfp277 <tm1> targeted mutation 1
loxP phage P1 loxP12loxP
loxP phage P1 loxP12loxP
neo neomycin resistance gene (E. coli)12 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • early cellular replicative senescence(MP:0008008)
    • Detailed phenotype data

    Ordering Information

    Donor DNABacteriophage loxP sites, Mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, SV40 PolyA signal, mouse Zfp277 genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PloS One., 5, e12373 (2010).
    DepositorAtsushi Iwama (Chiba University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data