Strain Data Sheet

RBRC05543

Strain Information

Image
BRC No.RBRC05543
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;B6129-Rest<tm1.1Yasu>
Former Common nameRest cKO, B6-Rest floxed
H-2 Haplotype
ES Cell lineV6.5 [(C57BL/6J x 129S4/SvJae)F1]
Background strain
Appearance
Strain developmentDeveloped by Yasuhiro Yamada, Gifu University Graduate School of Medicine / Center for iPS Cell Research and Application, Kyoto University. C57BL/6J and 129S4 mixed background. The neomycin resistant cassette was removed by Cre mediated recombination.
Strain descriptionRest, RE1-silencing transcription factor gene floxed mice. Exon 4 of Rest gene was replaced with a loxP-exon 4-IRES-GFP-loxP construct. Conditional Rest deficient mice can be generated by crossing with tissue-specific Cre mice.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesCell Stem Cell, 6, 10-15 (2010). 20085738

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
RestRE1-silencing transcription factor5Rest<tm1.1Yasu>targeted mutation 1.1, Yasuhiro Yamada

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)5

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)5internal ribosomal entry site from EMCV

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP5loxP

Ordering Information

Donor DNABacteriophage P1 loxP sites, Encephalomyocarditis virus IRES, A. victoria GFP cDNA, mouse Rest genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR, KYOTO UNIVERSITY at cira-keiyaku@cira.kyoto-u.ac.jp.
DepositorYasuhiro Yamada (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data