Strain Data Sheet

RBRC05543

Strain Information

Image
BRC No.RBRC05543
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;B6129-Rest<tm1.1Yasu>
Former Common nameRest cKO, B6-Rest floxed
H-2 Haplotype
ES Cell lineV6.5 [(C57BL/6J x 129S4/SvJae)F1]
Background strain
Appearance
Strain developmentDeveloped by Yasuhiro Yamada, Gifu University Graduate School of Medicine / Center for iPS Cell Research and Application, Kyoto University. C57BL/6J and 129S4 mixed background. The neomycin resistant cassette was removed by Cre mediated recombination.
Strain descriptionRest, RE1-silencing transcription factor gene floxed mice. Exon 4 of Rest gene was replaced with a loxP-exon 4-IRES-GFP-loxP construct. Conditional Rest deficient mice can be generated by crossing with tissue-specific Cre mice.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
Rest promotes the early differentiation of mouse ESCs but is not required for their maintenance.
Yamada Y, Aoki H, Kunisada T, Hara A
Cell Stem Cell, 6, 10-15 (2010). 20085738

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFP Green Fluorescent Protein (Aequorea victoria)5
IRES internal ribosomal entry site (EMCV)5
Rest
MGI:104897		RE1-silencing transcription factor5Rest<tm1.1Yasu>
MGI:4437069		targeted mutation 1.1, Yasuhiro Yamada
  • autosomal dominant nonsyndromic hearing loss 27(MedGEN)

  • fibromatosis, gingival, 5(MedGEN)

  • Wilms tumor 6(MedGEN)
    • loxP phage P1 loxP5loxP
    loxP phage P1 loxP5loxP

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal primordial germ cell apoptosis(MP:0011610)

  • decreased primordial germ cell number(MP:0008392)
    • Detailed phenotype data

    Ordering Information

    Donor DNABacteriophage P1 loxP sites, Encephalomyocarditis virus IRES, A. victoria GFP cDNA, mouse Rest genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR, KYOTO UNIVERSITY at cira-keiyaku@cira.kyoto-u.ac.jp.
    DepositorYasuhiro Yamada (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data