Strain Data Sheet

RBRC05549

Strain Information

Image
BRC No.RBRC05549
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129(Cg)-Capns1<tm1.1Otsu>
Former Common nameB6-Capns1 floxed
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Kinya Otsu, Osaka University Graduate School of Medicine in 2006. A loxP site was inserted upstream of exon 7 of Capns1 gene. A floxed neo cassette was inserted in downstream of exon 9. The PGK-neo cassette was removed by crossing with EIIa-cre mice. C57BL/6 and 129 mixed background.
Strain descriptionCapns1, calpain, small subunit 1 floxed mice. Exons 7-9 of Capns1 gene was flanked by loxP sites. Conditional Capns1 deficient mice can be generated by crossing with tissue-specific Cre mice. Homozygous mice for floxed allele show no obvious abnormality.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6]
References
Calpain protects the heart from hemodynamic stress.
Taneike M, Mizote I, Morita T, Watanabe T, Hikoso S, Yamaguchi O, Takeda T, Oka T, Tamai T, Oyabu J, Murakawa T, Nakayama H, Nishida K, Takeda J, Mochizuki N, Komuro I, Otsu K
J. Biol. Chem., 286, 32170-32177 (2011). 21795695

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Capns1
MGI:88266		calpain, small subunit 17Capns1<tm1.1Otsu>
MGI:5293309		targeted mutation 1.1, Kinya Otsu
loxP phage P1 loxP7loxP
loxP phage P1 loxP7loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal heart left ventricle morphology(MP:0003921)

  • abnormal myocardial fiber physiology(MP:0004215)

  • cardiac fibrosis(MP:0003141)

  • congestive heart failure(MP:0006138)

  • decreased cardiac muscle contractility(MP:0005140)
    • more 3 phenotypes
  • dilated heart left ventricle(MP:0002753)

  • increased lung weight(MP:0005630)

  • increased response of heart to induced stress(MP:0004485)
    • Detailed phenotype data

    Ordering Information

    Donor DNAbacteriophage P1 loxP sites, mouse Capns1 genomic DNA
    Research applicationCardiovascular Research
    Cre/loxP system
    covid19_progression
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 286, 32170-32177 (2011). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorKinya Otsu (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data