Strain Data Sheet

RBRC05550

Strain Information

Image
BRC No.RBRC05550
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Ighm<tm1Cgn> Tg(Aicda-cre)9Mbu Gt(ROSA)26Sor<tm1Sho>
Former Common namemuMT/Aicda-cre/R26R, B6-Ighm KO/AicdaCre/ROSA26-lacZ
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentmuMT : Klaus Rajewsky, Insitute for Genetics, University of Cologne. R26R : Philippe Soriano, Mount Sinal School of Medicine. Aicda-cre : Meinrad Busslinger. Research Institute of Molecular Pathology.
Strain descriptionThis strain has Ighm knockout (Ighm<tm1Cgn>), AID-Tg (Tg(Aicda-cre)9Mbu) and ROSA26 lacZ reporter (Gt(ROSA)26Sor<tm1Sho>) alleles. Immunodeficient.
Colony maintenanceIghm : Homozygote x Homozygote ; Tg : Carrier x Carrier ; ROSA26 : Homozygote x Homozygote
References
A B cell-deficient mouse by targeted disruption of the membrane exon of the immunoglobulin mu chain gene.
Kitamura D, Roes J, Kuhn R, Rajewsky K
Nature, 350, 423-436 (1991). 1901381

Improved reporter strain for monitoring Cre recombinase-mediated DNA excisions in mice.
Mao X, Fujiwara Y, Orkin S H
Proc. Natl. Acad. Sci. USA, 96, 5037-5042 (1999). 10220414

Instructive role of the transcription factor E2A in early B lymphopoiesis and germinal center B cell development.
Kwon K, Hutter C, Sun Q, Bilic I, Cobaleda C, Malin S, Busslinger M
Immunity, 28, 751-762 (2008). 18538592

Activation-induced cytidine deaminase expression in CD4+ T cells is associated with a unique IL-10-producing subset that increases with age.
Qin H, Suzuki K, Nakata M, Chikuma S, Izumi N, Huong le T, Maruya M, Fagarasan S, Busslinger M, Honjo T, Nagaoka H
PLoS One, 6, e29141 (2011). 22216188

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ighm
MGI:96448		immunoglobulin heavy constant mu12Ighm<tm1Cgn>
MGI:1857187		targeted mutation 1, University of Cologne
Gt(ROSA)26Sor
MGI:104735		gene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1Sho>
MGI:1861931		targeted mutation 1, Stuart Orkin
loxP phage P1 loxP6loxP
loxP phage P1 loxP6loxP
neo neomycin resistance gene (E. coli)6 mouse phosphoglycerate kinase promoter (PGK promoter)
CD2 CD2 (human)UNCD2
Ires internal ribosomal entry site (EMCV)UN
cre Phage P1 Cre recombinaseUNcre mouse Aicda promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal B cell differentiation(MP:0002144)

  • abnormal B lymphocyte antigen presentation(MP:0002453)

  • abnormal CD4-positive, alpha-beta T cell physiology(MP:0005463)

  • abnormal CD8 positive, alpha-beta intraepithelial T cell morphology(MP:0008401)

  • abnormal T cell number(MP:0006387)
    • more 50 phenotypes
  • abnormal T cell proliferation(MP:0005094)

  • abnormal antigen presentation(MP:0001835)

  • abnormal cardiovascular system physiology(MP:0001544)

  • abnormal compact bone morphology(MP:0003797)

  • abnormal cytokine secretion(MP:0003009)

  • abnormal humoral immune response(MP:0001800)

  • abnormal intestinal epithelium morphology(MP:0000488)

  • abnormal lymphatic vessel morphology(MP:0001879)

  • abnormal mature B cell morphology(MP:0008171)

  • abnormal metallophilic macrophage morphology(MP:0008241)

  • abnormal pancreatic islet morphology(MP:0005215)

  • abnormal respiratory system physiology(MP:0002133)

  • abnormal response to infection(MP:0005025)

  • abnormal spleen B cell follicle morphology(MP:0008470)

  • abnormal spleen marginal sinus morphology(MP:0002363)

  • abnormal spleen marginal zone macrophage morphology(MP:0008240)

  • abnormal spleen morphology(MP:0000689)

  • abnormal trabecular bone morphology(MP:0000130)

  • absent B cells(MP:0008071)

  • absent follicular dendritic cells(MP:0008201)

  • absent immature B cells(MP:0008213)

  • absent mature B cells(MP:0008212)

  • arrested B cell differentiation(MP:0001802)

  • decreased B cell number(MP:0005017)

  • decreased CD4-positive, alpha-beta T cell number(MP:0008075)

  • decreased CD8-positive, alpha-beta T cell number(MP:0008079)

  • decreased IgA level(MP:0001807)

  • decreased IgE level(MP:0002492)

  • decreased IgG level(MP:0001805)

  • decreased IgG1 level(MP:0008495)

  • decreased IgM level(MP:0001806)

  • decreased T cell proliferation(MP:0005095)

  • decreased bone mineral density(MP:0000063)

  • decreased compact bone thickness(MP:0000135)

  • decreased compact bone volume(MP:0010965)

  • decreased dendritic cell number(MP:0008127)

  • decreased immunoglobulin level(MP:0002460)

  • decreased spleen weight(MP:0004953)

  • decreased susceptibility to autoimmune diabetes(MP:0004804)

  • decreased susceptibility to injury(MP:0005166)

  • decreased susceptibility to type IV hypersensitivity reaction(MP:0005616)

  • decreased trabecular bone thickness(MP:0009346)

  • immune system phenotype(MP:0005387)

  • increased lymphoma incidence(MP:0012431)

  • increased osteoclast cell number(MP:0004984)

  • increased susceptibility to autoimmune diabetes(MP:0004803)

  • increased susceptibility to induced arthritis(MP:0003724)

  • increased susceptibility to parasitic infection(MP:0005027)

  • insulitis(MP:0004031)

  • respiratory system phenotype(MP:0005388)
    • Detailed phenotype data

    Ordering Information

    Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neomycin phosphotransferase, polyA, mouse Ighm genomic DNA, mouse Aicda genomic DNA (BAC clone RP24-68I7), SV40 nuclear localization sequence, Phage P1 Cre recombinase, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), human CD2 cDNA, SV40 polyA signal, retrovirus LTR, adenovirus major late transcript splice acceptor (SA) sequence, Phage P1 loxP sites, E.coli lacZ, E. coli neomycin phosphotransferase, bovine growth hormone polyA, mouse ROSA26 locus genomic DNA
    Research applicationCre/loxP system
    Cre driver
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One, 6, e29141 (2011).The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
    The BIOLOGICAL RESOURCE contains Tg(Aicda-re)9Mbu and Gt(ROSA)26Sortm1Sho. Before executing the MTA between RIKEN BRC and the RECIPIENT, the RECIPIENT shall contact Dr. Meinrad Busslinger, Research Institute of Molecular Pathology and shall execute the material transfer agreement with Research Institute of Molecular Pathology to use Tg(Aicda-cre)9Mbu. The RECIPIENT shall obtain the prior written consent of Dr. Philippe Soriano, Mount Sinai School of Medicine to use Gt(ROSA)26Sortm1Sho.
    DepositorTasuku Honjo (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Honjo Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data