Strain Data Sheet

RBRC05552

Strain Information

Image
BRC No.RBRC05552
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain name129S6.129S(Cg)-Grm6<tm1Nak>
Former Common name129-mGluR6 KO, 129-Grm6 KO
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strain129S6/SvEvTac
Appearance
Strain developmentDeveloped by Shigetada Nakanishi and Takahisa Furukawa, Osaka Bioscience Institute in 2012. The mutant mice were generated by backcrossing to 129S6/SvEvTac from B6-Grm6-lacZ Tg/Grm6 KO mice (RBRC01353).
Strain descriptionmGluR6 (Grm6) knockout mice. 126 background. 1.2 kb sequence encoding part of the transmembrane region of Grm6 gene was replaced with a neomycin resistance cassette.
Colony maintenanceHeterozygote x Homozygote [or Crossing to 129S6/SvEvTac]
References
Specific deficit of the ON response in visual transmission by targeted disruption of the mGluR6 gene.
Masu M, Iwakabe H, Tagawa Y, Miyoshi T, Yamashita M, Fukuda Y, Sasaki H, Hiroi K, Nakamura Y, Shigemoto R, et a l
Cell, 80, 757-765 (1995). 7889569

Immunohistological studies of metabotropic glutamate receptor subtype 6-deficient mice show no abnormality of retinal cell organization and ganglion cell maturation.
Tagawa Y, Sawai H, Ueda Y, Tauchi M, Nakanishi S
J. Neurosci., 19, 2568-2579 (1999). 10087070

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Grm6
MGI:1351343		glutamate receptor, metabotropic 611Grm6<tm1Nak>
MGI:1857985		targeted mutation 1, Shigetada Nakanishi
  • congenital stationary night blindness 1B(DisGeNET, MedGEN)
    • neo neomycin resistance gene (E. coli)11 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal eye electrophysiology(MP:0005551)

  • abnormal synaptic transmission(MP:0003635)

  • behavior/neurological phenotype(MP:0005386)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli Neomycin resistance gene, Herpes simplex virus thymidine kinase promoter (HSV tk promoter), Mouse Grm6 genomic DNA
    Research applicationNeurobiology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell, 80, 757-765 (1995). J. Neurosci., 19, 2568-2579 (1999).
    DepositorDrs. Shigetada Nakanishi and Takahisa Furukawa (Osaka Bioscience Institute)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Orlandi C, Omori Y, Wang Y, Cao Y, Ueno A, Roux MJ, Condomitti G, de Wit J, Kanagawa M, Furukawa T, Martemyanov KA.
    Transsynaptic Binding of Orphan Receptor GPR179 to Dystroglycan-Pikachurin Complex Is Essential for the Synaptic Organization of Photoreceptors.
    Cell Rep 25(1) 130-145.e5(2018) 30282023