Strain Data Sheet

RBRC05553

Strain Information

Image
BRC No.RBRC05553
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Pmaip1<tm1Ttg>
Former Common nameB6-Noxa KO
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Tsukasa Shibue and Tadatsugu Taniguchi, Graduate School of Medicine, University of Tokyo in 2003. E14.1 ES cell derived. The mutant mice were backcrossed to C57BL/6J.
Strain descriptionNoxa (Pmaip1) gene knockout mice. A 2.4 kb genomic region including exons 1 and 2 of the Pmaip1 gene was replaced with a neomycin selection cassette. Homozygous mutant mice are resistant to X-ray irradiation-induced gastrointestinal death.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
Integral role of Noxa in p53-mediated apoptotic response.
Shibue T, Takeda K, Oda E, Tanaka H, Murasawa H, Takaoka A, Morishita Y, Akira S, Taniguchi T, Tanaka N
Genes Dev., 17, 2233-2238 (2003). 12952892

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Pmaip1
MGI:1930146		phorbol-12-myristate-13-acetate-induced protein 118Pmaip1<tm1Ttg>
MGI:2678372		targeted mutation 1, Tadatsugu Taniguchi
neo neomycin resistance gene (E. coli)18

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • decreased cellular sensitivity to X-ray irradiation(MP:0004587)

  • decreased cellular sensitivity to ultraviolet irradiation(MP:0008411)

  • decreased mortality induced by ionizing radiation(MP:0003010)

  • decreased sensitivity to induced cell death(MP:0008944)

  • neoplasm(MP:0002006)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli Neomycin resistance gene
    Research application
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT shall inform the DEPOSITOR about the purpose of the use of the BIOLOGICAL RESOURCE and shall limit the use within the scope of the purpose described in this Agreement. The RECIPIENT shall inform the DEPOSITOR about the results obtained by the use of the BIOLOGICAL RESOURCE.
    DepositorTadatsugu Taniguchi (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data