Strain Data Sheet

RBRC05554

Strain Information

Image
BRC No.RBRC05554
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Egfl6<tm1Ksek>
Former Common nameMAEG delta RGD, B6-Egfl6 KO, B6-Egfl6/dRGD
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Kiyotoshi Sekiguchi, Institute for Protein Research, Osaka University in 2012. 129 derived ES cells were used. The mutant mice were crossed to C57BL/6J.
Strain descriptionMAEG (Egfl6) RDG motif (Arg-Gly-Asp knockout mice. Exon 9 and 10 encoding RDG motif of the Egfl6 gene were replaced with PGK-neomycin resistant gene. X linked.
Colony maintenanceHeterozygote x Wild-type [C57BL/6J]
References
Expression of MAEG, a novel basement membrane protein, in mouse hair follicle morphogenesis.
Osada A, Kiyozumi D, Tsutsui K, Ono Y, Weber C N, Sugimoto N, Imai T, Okada A, Sekiguchi K
Exp. Cell Res., 303, 148-159 (2005). 15572035

Basement membrane assembly of the integrin alpha8beta1 ligand nephronectin requires Fraser syndrome-associated proteins.
Kiyozumi D, Takeichi M, Nakano I, Sato Y, Fukuda T, Sekiguchi K
J. Cell Biol., 197(5):677-689 (2012). 22613833

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Egfl6
MGI:1858599		EGF-like-domain, multiple 6XEgfl6<tm1Ksek> targeted mutation 1, Kiyotoshi Sekiguchi
neo neomycin resistance gene (E. coli)X mouse phosphoglycerate kinase promoter (PGK promoter)
SV40 polyA signalX

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Phosphoglycerate kinase promoter, E. coli Neomycin resistance gene, SV40 PolyA signal, mouse Egfl6 genomic DNA
    Research application
    Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorKiyotoshi Sekiguchi (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data