Strain Data Sheet

RBRC05555

Strain Information

Image
BRC No.RBRC05555
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;B6129-Ezh2<tm1Yugo>/Hko
Former Common nameB6-Ezh2 floxed
H-2 Haplotype
ES Cell lineM1 [(C57BL/6 x 129)F1]
Background strain
Appearance
Strain developmentDeveloped by Haruhiko Koseki and Jun Shinga, RIKEN Center for Allergy and Immunology in 2009. M1 ES cells derived from (C57BL/6 x 129) F1 were used. The mutant mice were crossed to C57BL/6J.
Strain descriptionEzh2 floxed mice. A floxed neo cassette was inserted upstream of exon 18 and an additional loxP was inserted downstream of exon 19 of Ezh2 gene. Conditional Ezh2 deficient mice can be generated by crossing with tissue-specific Cre mice.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
References
Polycomb limits the neurogenic competence of neural precursor cells to promote astrogenic fate transition.
Hirabayashi Y, Suzki N, Tsuboi M, Endo T A, Toyoda T, Shinga J, Koseki H, Vidal M, Gotoh Y
Neuron, 63, 600-613 (2009). 19755104

Dependency on the polycomb gene Ezh2 distinguishes fetal from adult hematopoietic stem cells.
Mochizuki-Kashio M, Mishima Y, Miyagi S, Negishi M, Saraya A, Konuma T, Shinga J, Koseki H, Iwama A
Blood, 118, 6553-6561 (2011). 22042701

Health Report

Examination Date / Room / Rack2024/10/07Room:4-ARack:FSentinel mouse program
2024/08/19Room:4-ARack:FSentinel mouse program
2024/05/20Room:4-ARack:FSentinel mouse program
2024/02/19Room:4-ARack:FSentinel mouse program
2023/11/20Room:4-ARack:FSentinel mouse program
2023/08/28Room:4-ARack:FAdditional tests
2023/08/21Room:4-ARack:FSentinel mouse program
2023/06/19Room:4-ARack:FAdditional tests
2023/05/22Room:4-ARack:FSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ezh2
MGI:107940		enhancer of zeste 2 polycomb repressive complex 2 subunit6Ezh2<tm1Yugo>
MGI:4412411		targeted mutation 1, Yukiko Gotoh
  • Weaver syndrome(DisGeNET, MedGEN)
    • loxP phage P1 loxP6loxP
    loxP phage P1 loxP6loxP
    loxP phage P1 loxP6loxP
    neo neomycin resistance gene (E. coli)6 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal neuron differentiation(MP:0009937)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 LoxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E.coli Neomycin resistance gene, mouse Ezh2 genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsThe RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorHaruhiko Koseki (RIKEN)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Fukui Y, Hirota Y, Aikawa S, Sakashita A, Shimizu-Hirota R, Takeda N, Ishizawa C, Iida R, Kaku T, Hirata T, Hiraoka T, Akaeda S, Matsuo M, Osuga Y.
    The EZH2-PRC2-H3K27me3 axis governs the endometrial cell cycle and differentiation for blastocyst invasion.
    Cell Death Dis 14(5) 320(2023) 37198149
    Kunanopparat A, Leelahavanichkul A, Visitchanakun P, Kueanjinda P, Phuengmaung P, Sae-Khow K, Boonmee A, Benjaskulluecha S, Palaga T, Hirankarn N.
    The Regulatory Roles of Ezh2 in Response to Lipopolysaccharide (LPS) in Macrophages and Mice with Conditional Ezh2 Deletion with LysM-Cre System.
    Int J Mol Sci 24(6) (2023) 36982437
    Phuengmaung P, Khiewkamrop P, Makjaroen J, Issara-Amphorn J, Boonmee A, Benjaskulluecha S, Ritprajak P, Nita-Lazar A, Palaga T, Hirankarn N, Leelahavanichkul A.
    Less Severe Sepsis in Cecal Ligation and Puncture Models with and without Lipopolysaccharide in Mice with Conditional Ezh2-Deleted Macrophages (LysM-Cre System).
    Int J Mol Sci 24(10) (2023) 37239864
    Ohka F, Shinjo K, Deguchi S, Matsui Y, Okuno Y, Katsushima K, Suzuki M, Kato A, Ogiso N, Yamamichi A, Aoki K, Suzuki H, Sato S, Arul Rayan N, Prabhakar S, Göke J, Shimamura T, Maruyama R, Takahashi S, Suzumura A, Kimura H, Wakabayashi T, Zong H, Natsume A, Kondo Y.
    Pathogenic Epigenetic Consequences of Genetic Alterations in IDH-Wild-Type Diffuse Astrocytic Gliomas.
    Cancer Res 79(19) 4814-4827(2019) 31431463