Strain Data Sheet

RBRC05558

Strain Information

Image
BRC No.RBRC05558
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain name129(Cg)-Rax<tm1.1Tfur>
Former Common name129-Rax floxed
H-2 Haplotype
ES Cell lineTC1 [129S6/SvEvTac]
Background strain129S6/SvEvTac
Appearance
Strain developmentDeveloped by Takahisa Furukawa, Osaka Bioscience Institute in 2006. 129 background. A Frt-flanked PGK-neo selection cassette was removed by crossing with FLP1 mice (B6;SJL-Tg(ACTFLPe)9205Dym/J).
Strain descriptionRax gene floxed mice. Exons 1 and 2 of the Rax gene were flanked by loxP sites. Conditional Rax deficient mice can be generated by crossing with tissue-specific Cre mice.
Colony maintenanceHomozygote x Homozygote [or Crossing to 126S6/SvEvTac]
References
An essential role for RAX homeoprotein and NOTCH-HES signaling in Otx2 expression in embryonic retinal photoreceptor cell fate determination.
Muranishi Y, Terada K, Inoue T, Katoh K, Tsujii T, Sanuki R, Kurokawa D, Aizawa S, Tamaki Y, Furukawa T
J. Neurosci., 31, 16792-16807 (2011). 22090505

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site18Frt
Rax
MGI:109632
retina and anterior neural fold homeobox18Rax<tm1.1Tfur>
MGI:5302230
targeted mutation 1.1, Takahisa Furukawa
  • isolated microphthalmia 3(MedGEN)
  • loxP phage P1 loxP18loxP
    loxP phage P1 loxP18loxP

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • retina hypoplasia(MP:0002984)
  • Detailed phenotype data

    Ordering Information

    Donor DNABacteriophage P1 loxP sites, Saccharomyces cerevisiae frt site, mouse Rax genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 31, 16792-16807 (2011). Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University).
    DepositorTakahisa Furukawa (Osaka Bioscience Institute)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Rohde K, Bering T, Furukawa T, Rath MF.
    A modulatory role of the Rax homeobox gene in mature pineal gland function: Investigating the photoneuroendocrine circadian system of a Rax conditional knockout mouse.
    J Neurochem 143(1) 100-111(2017) 28675567