Strain Data Sheet

RBRC05559

Strain Information

Image
BRC No.RBRC05559
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Crx-cre/ERT2)1Tfur
Former Common name129S6;B6C3-Tg(Crx-cre/ERT2)1Tfur, 129-CrxCreERT2, 129-CrxCreER Tg
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Takahisa Furukawa, Osaka Bioscience Institute in 2008. The transgene was injected into the pronuclei of B6C3F1 fertilized eggs.
Strain descriptionCrx-creERT2 transgenic mice. This transgenic mouse expresses tamoxifen-inducible cre recombinase gene in retinal photoreceptor cells and progenitor cells under the control of the mouse Crx promoter.
Colony maintenanceCarrier x Noncarrier [C57BL/6NCrlCrlj]
References
An essential role for RAX homeoprotein and NOTCH-HES signaling in Otx2 expression in embryonic retinal photoreceptor cell fate determination.
Muranishi Y, Terada K, Inoue T, Katoh K, Tsujii T, Sanuki R, Kurokawa D, Aizawa S, Tamaki Y, Furukawa T
J. Neurosci., 31, 16792-16807 (2011). 22090505

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
ERT2 estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)UNERT2
cre Phage P1 Cre recombinaseUNcre
MGI:5302219		Crx2K promoter
SV40 polyA signalUN

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Crx promoter, phage P1 Cre recombinase gene, SV40 polyA signal, human Estrogen receptor binding domain (ERT2)
    Research applicationCre/loxP system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 31, 16792-16807 (2011). Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University).
    DepositorTakahisa Furukawa (Osaka Bioscience Institute)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Cre-ERT(2): NOTICE TO RECIPIENT
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month May 2013

    BRC mice in Publications

    No Data