Strain Data Sheet

RBRC05562

Strain Information

Image
BRC No.RBRC05562
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Crx-rtTA)G2Tfur
Former Common nameB6-Crx-rtTA Tg#G2
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Takahisa Furukawa, Osaka Bioscience Institute in 2010. The Crx-rtTA transgene was injected into the pronuclei of B6C3F1 fertilized eggs.
Strain descriptionCrx-rtTA transgenic mice. These transgenic mice express reverse tetracycline-controlled transactivator (rtTA) gene under the control of the mouse Crx promoter.
Colony maintenanceCarrier x Noncarrier [C57BL/6NCrlCrlj]
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
rtTAE. coli Reverse tetracycline transactivator (Mutated tetrepressor DNA-binding domain and the transactivation domain of VP-16)UNrtTAE. coli tet repressor (TetR), herpes simplex virus (HSV)VP16 activation domainmouse Crx promoter

Ordering Information

Donor DNAMouse Crx genomic DNA, VP-16 Reverse tetracycline transactivator (Mutated tetrepressor DNA-binding domain and transactivation domain), SV40 polyA signal
Research applicationTet system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University).
DepositorTakahisa Furukawa (Osaka Bioscience Institute)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data