Strain Data Sheet

RBRC05582

Strain Information

Image
BRC No.RBRC05582
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Ikzf3<tm1(KOMP)Vlcg>/CB3
Former Common name
H-2 Haplotype
ES Cell lineVGB6 [C57BL/6NTac]
Background strainC57BL/6NTac
Appearance
Strain development
Strain description
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ikzf3
MGI:1342542		IKAROS family zinc finger 311Ikzf3<tm1(KOMP)Vlcg>
MGI:5008103		targeted mutation 1, Velocigene
lacZ beta-galactosidase (E. coli)11
loxP phage P1 loxP11loxP
loxP phage P1 loxP11loxP
neo neomycin resistance gene (E. coli)11 human ubiquitin C promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • decreased CD4-positive NK T cell number(MP:0013519)

  • decreased Ly6C-positive immature NK cell number(MP:0013669)

  • decreased Ly6C-positive mature NK cell number(MP:0013675)

  • decreased large unstained cell number(MP:0012361)

  • decreased leukocyte cell number(MP:0000221)
    • more 9 phenotypes
  • decreased lymphocyte cell number(MP:0005016)

  • decreased memory-marker CD4-negative NK T cell number(MP:0013513)

  • decreased memory-marker CD4-positive NK T cell number(MP:0013522)

  • immune system phenotype(MP:0005387)

  • increased CD11b-high dendritic cell number(MP:0013648)

  • increased CD4-negative NK T cell number(MP:0013511)

  • increased effector memory CD8-positive, alpha-beta T cell number(MP:0010850)

  • increased monocyte cell number(MP:0000220)

  • increased neutrophil cell number(MP:0000219)
    • Detailed phenotype data

    Ordering Information

    Donor DNAVelocigene cassette ZEN-Ub1(E. coli lacZ, pA, Phage P1 loxP sites, human ubiquitin C promoter, E. coli neo, pA), mouse Ikzf3 genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and Conditions
    DepositorRIKEN BRC (RIKEN BRC)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Read KA, Jones DM, Pokhrel S, Hales EDS, Varkey A, Tuazon JA, Eisele CD, Abdouni O, Saadey A, Leonard MR, Warren RT, Powell MD, Boss JM, Hemann EA, Yount JS, Xin G, Ghoneim HE, Lio CJ, Freud AG, Collins PL, Oestreich KJ.
    Aiolos represses CD4+ T cell cytotoxic programming via reciprocal regulation of TFH transcription factors and IL-2 sensitivity.
    Nat Commun 14(1) 1652(2023) 36964178