Strain Data Sheet

RBRC05582

Strain Information

Image
BRC No.RBRC05582
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Ikzf3<tm1(KOMP)Vlcg>/CB3
Former Common name
H-2 Haplotype
ES Cell lineVGB6 [C57BL/6NTac]
Background strainC57BL/6NTac
Appearance
Strain development
Strain description
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ikzf3IKAROS family zinc finger 311Ikzf3<tm1(KOMP)Vlcg>targeted mutation 1, Velocigene

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)11

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)11human ubiquitin C promoter

Ordering Information

Donor DNAVelocigene cassette ZEN-Ub1(E. coli lacZ, pA, Phage P1 loxP sites, human ubiquitin C promoter, E. coli neo, pA), mouse Ikzf3 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and Conditions
DepositorRIKEN BRC (RIKEN BRC)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Read KA, Jones DM, Pokhrel S, Hales EDS, Varkey A, Tuazon JA, Eisele CD, Abdouni O, Saadey A, Leonard MR, Warren RT, Powell MD, Boss JM, Hemann EA, Yount JS, Xin G, Ghoneim HE, Lio CJ, Freud AG, Collins PL, Oestreich KJ.
Aiolos represses CD4+ T cell cytotoxic programming via reciprocal regulation of TFH transcription factors and IL-2 sensitivity.
Nat Commun 14(1) 1652(2023) 36964178