Strain Data Sheet

RBRC05584

Strain Information

Image
BRC No.RBRC05584
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Ikzf5<tm1(KOMP)Wtsi>/C06
Former Common name
H-2 Haplotype
ES Cell lineJM8A3.N1 [C57BL/6N-A<tm1Brd>]
Background strainC57BL/6NTac
Appearance
Strain development
Strain description
Colony maintenance
References
Agouti C57BL/6N embryonic stem cells for mouse genetic resources.
Pettitt S J, Liang Q, Rairdan X Y, Moran J L, Prosser H M, Beier D R, Lloyd K C, Bradley A, Skarnes W C
Nature Methods 6, 493-495 (2009). 19525957

The mammalian gene function resource: the International Knockout Mouse Consortium.
Bradley A, Anastassiadis K, Ayadi A, Battey J F, Bell C, Birling M C, Bottomley J, Brown S D, Burger A, Bult C J, Bushell W, Collins F S, Desaintes C, Doe B, Economides A, Eppig J T, Finnell R H, Fletcher C, Fray M, Frendewey D, Friedel R H, Grosveld F G, Hansen J, Herault Y, Hicks G, Horlein A, Houghton R, Hrabe de Angelis M, Huylebroeck D, Iyer V, de Jong P J, Kadin J A, Kaloff C, Kennedy K, Koutsourakis M, Lloyd K C, Marschall S, Mason J, McKerlie C, McLeod M P, von Melchner H, Moore M, Mujica A O, Nagy A, Nefedov M, Nutter L M, Pavlovic G, Peterson J L, Pollock J, Ramirez-Solis R, Rancourt D E, Raspa M, Remacle J E, Ringwald M, Rosen B, Rosenthal N, Rossant J, Ruiz Noppinger P, Ryder E, Schick J Z, Schnutgen F, Schofield P, Seisenberger C, Selloum M, Simpson E M, Skarnes W C, Smedley D, Stanford W L, Stewart A F, Stone K, Swan K, Tadepally H, Teboul L, Tocchini-Valentini G P, Valenzuela D, West A P, Yamamura K, Yoshinaga Y, Wurst W
Mamm. Genome, 23(9-10), 580-586 (2012). 22968824

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
En2 En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)7En2
Frt yeast FRT (flippase recombination target) site7Frt
Frt yeast FRT (flippase recombination target) site7Frt
Ikzf5
MGI:1914393		IKAROS family zinc finger 57Ikzf5<tm1(KOMP)Wtsi>
MGI:4419517		targeted mutation 1, Wellcome Trust Sanger Institute
  • thrombocytopenia 7(MedGEN)
    • Ires internal ribosomal entry site (EMCV)7
    lacZ beta-galactosidase (E. coli)7
    loxP phage P1 loxP7loxP
    loxP phage P1 loxP7loxP
    neo neomycin resistance gene (E. coli)7 human beta-actin promoter
    SV40 polyA signal7

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • decreased effector memory CD8-positive, alpha-beta T cell number(MP:0010851)

  • increased or absent threshold for auditory brainstem response(MP:0011967)
    • Detailed phenotype dataPhenopub Link

    Ordering Information

    Donor DNAyeast FRT (flipase recombination target) sites, En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, SV40 polyadenylation site, phage P1 loxP sites, hBactP (human beta actin promoter), E. coli neo, mouse Ikzf5 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsThe mice were originally created by the National Institutes of Health (NIH) Knock-Out Mouse Program (KOMP). The RECIPIENT is free to publish, present, display, disclose or seek patent or other intellectual property protection on any inventions arising from the use of the KOMP Materials, with no restrictions or obligations of any kind, so long as the RECIPIENT uses reasonable efforts to credit or otherwise acknowledge the KOMP as the creator of the KOMP Materials.
    The RECIPIENT shall use the following acknowledgement phrase in any resulting publication:
    The Ikzf5 KO mice were obtained from the RIKEN BioResource Center which generated the The Ikzf5 KO mice using knockout ES cells provided by the KOMP Repository and is a member of the International Mouse Phenotyping Consortium (IMPC). Associated primary phenotypic information may be found at www.mousephenotype.org. When details of the alleles have been published, the RECIPIENT shall include the following references in publications:
    Bradley A, Anastassiadis K, Ayadi A, Battey JF, Bell C, Birling M-C, Bottomley J, Brown SD, Burger A, Bult CJ, Bushell W, Collins FS, Desaintes C, Doe B, Economides A, Eppig JT, Finnell RH, Fletcher C, Fray M, Frendewey D, et al. (2012) The mammalian gene function resource: the international knockout mouse consortium. Mamm. Genome, 23(9-10), 580-586. Pettitt SJ, Liang Q, Rairdan XY, Moran JL, Prosser HM, Beier DR, Lloyd KC, Bradley A & Skarnes WC (2009) Agouti C57BL/6N embryonic stem cells for mouse genetic resources. Nature methods, 6(7), 493-495.
    DepositorRIKEN BRC (RIKEN BRC)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data