Strain Data Sheet

RBRC05589

Strain Information

Image
BRC No.RBRC05589
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Trpc5<tm1a(KOMP)Wtsi>/B02
Former Common name
H-2 Haplotype
ES Cell lineJM8.N4 [C57BL/6N]
Background strainC57BL/6NTac
Appearance
Strain development
Strain description
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) siteXFrt
Frt yeast FRT (flippase recombination target) siteXFrt
Ires internal ribosomal entry site (EMCV)X
SA Splice acceptorXSA
Trpc5
MGI:109524		transient receptor potential cation channel, subfamily C, member 5XTrpc5<tm1a(KOMP)Wtsi>
MGI:4364838		targeted mutation 1a, Wellcome Trust Sanger Institute
lacZ beta-galactosidase (E. coli)X
loxP phage P1 loxPXloxP
loxP phage P1 loxPXloxP
loxP phage P1 loxPXloxP
neo neomycin resistance gene (E. coli)X hBactP (human beta actin promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAyeast FRT (flipase recombination target) sites, En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, SV40 polyadenylation site, phage P1 loxP sites, hBactP (human beta actin promoter), E. coli neo, mouse Trpc5 genomic DNA
    Research application
    Specific Term and ConditionsThe RECIPIENT should acknowledge the KOMP, RIKEN BRC and the International Mouse Phenotyping Consortium as the original creator of the targeted mouse ES cells and the provider of the knockout mice, respectively.
    DepositorRIKEN BRC (RIKEN BRC)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data