Strain Data Sheet

RBRC05599

Strain Information

Image
BRC No.RBRC05599
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129(Cg)-Vps41<tm1.1Ywd>
Former Common namemVam2 null, B6-Vps41 KO
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Yoh Wada, Institute of Scientific and Industrial Research, Osaka University. An m Vam2-floxNeo allele, containing the FRT-loxP-Npt-FRT-loxP in intron 13, and the third loxP sequence in intron 17, was introduced into the mouse genome by R1 ES cell mediated gene replacement. The modified allele was converted to mVam- (Vps41<tm1Ywd>) by crossing with FLP and Cre mice (B6;SJL-Tg(ACTFLPe)9205Dym/J and FVB/N-Tg(EIIa-cre)C5379Lmgd/J). The mutant mice were cross to C57BL/6.
Strain descriptionmVam2 (Vps41) knockout mice. Exons 14 to 18 of the mVam2 gene were removed. Homozygous mutant mice are embryonic lethal (E7.5-8.5).
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJmsSlc]
References
Simple and straightforward construction of a mouse gene targeting vector using in vitro transposition reactions.
Aoyama M, Agari K, Sun-Wada G H, Futai M, Wada Y
Nucleic Acids Res., 33, e52 (2005). 15784610

Spatial restriction of bone morphogenetic protein signaling in mouse gastrula through the mVam2-dependent endocytic pathway.
Aoyama M, Sun-Wada G H, Yamamoto A, Yamamoto M, Hamada H, Wada Y
Dev. Cell, 22, 1163-1175 (2012). 22698281

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site13Frt
Vps41
MGI:1929215		vacuolar protein sorting 41 (yeast)13Vps41 <tm1.1Ywd>
MGI:5425109		targeted mutation 1.1, Yoh Wada
  • spinocerebellar ataxia, autosomal recessive 29(MedGEN)
    • loxP phage P1 loxP13loxP

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal egg cylinder morphology(MP:0003085)

  • abnormal embryonic tissue morphology(MP:0002085)

  • abnormal endoderm development(MP:0001685)

  • abnormal germ layer development(MP:0001674)

  • abnormal lateral plate mesoderm morphology(MP:0010117)
    • more 5 phenotypes
  • abnormal mesoderm development(MP:0001680)

  • abnormal vesicle-mediated transport(MP:0008546)

  • embryo phenotype(MP:0005380)

  • embryonic lethality between somite formation and embryo turning, complete penetrance(MP:0011097)

  • increased ectoderm apoptosis(MP:0003895)
    • Detailed phenotype data

    Ordering Information

    Donor DNAEscherichia coli Tn7 Right border and left border, Bacteriophage P1 loxP site, Saccharomyces cerevisiae Frt site, Escherichia coli Tn5 Outer elements, mouse Vps41 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Metabolism Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or research in non-profit organization is requested to enter into a Material Transfer Agreement with Osaka University.
    DepositorYoh Wada (Osaka University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data