Strain Data Sheet

RBRC05601

Strain Information

Image
BRC No.RBRC05601
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129(Cg)-Rab7<tm1.2Ywd>
Former Common nameRab7 null, B6-rAB7 KO
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Yoh Wada, Institute of Scientific and Industrial Research, Osaka University. An Rab7 targeted allele, containing a loxP site in upstream of exon 2 and a RT-loxP-Neo-FRT-loxP cassette in downstream of exon 13, was introduced into the mouse genome by R1 ES cell mediated gene replacement. The modified allele was converted to Rab7- (Rab7<tm1.1Ywd>) by crossing with FLP and Cre mice (B6;SJL-Tg(ACTFLPe)9205Dym/J and FVB/N-Tg(EIIa-cre)C5379Lmgd/J). The mutant mice were cross to C57BL/6.
Strain descriptionRab7 gene knockout mice. Exons 2 and 3 of the Rab7 gene were removed. Homozygous mutant mice are embryonic lethal.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJmsSlc]
References
Spatial restriction of bone morphogenetic protein signaling in mouse gastrula through the mVam2-dependent endocytic pathway.
Aoyama M, Sun-Wada G H, Yamamoto A, Yamamoto M, Hamada H, Wada Y
Dev. Cell, 22, 1163-1175 (2012). 22698281

Delivery of endosomes to lysosomes via microautophagy in the visceral endoderm of mouse embryos.
Kawamura N, Sun-Wada G H, Aoyama M, Harada A, Takasuga S, Sasaki T, Wada Y
Nat. Commun., 3, 1071 (2012). 22990867

Rab7-Mediated Endocytosis Establishes Patterning of Wnt Activity through Inactivation of Dkk Antagonism.
Nobuyuki Kawamura, Katsuyoshi Takaoka, Hiroshi Hamada, Anna-Katerina Hadjantonakis, Ge-Hong Sun-Wada, Yoh Wada
Cell Rep., 31(10):107733 (2022). 32521258

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site6Frt
Rab7
MGI:105068		RAB7, member RAS oncogene family6Rab7 <tm1.2Ywd>
MGI:5437841		targeted mutation 1.2, Yoh Wada
  • Charcot-Marie-Tooth disease type 2B(DisGeNET, MedGEN)
    • loxP phage P1 loxP6loxP

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAEscherichia coli Tn7 Right border and left border, Bacteriophage P1 LoxP site, Saccharomyces cerevisiae Frt site, Escherichia coli Tn5 Outer elements, mouse Rab7 genomic DNA
    Research applicationCell Biology Research
    Cre/loxP system
    FLP/frt system
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or research in non-profit organization is requested to enter into a Material Transfer Agreement with Osaka University.
    DepositorYoh Wada (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data