Strain Data Sheet

RBRC05637

Strain Information

Image
BRC No.RBRC05637
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6D2;129S2-Igsf8<tm1.1Osb>
Former Common nameIgsf8 floxed
H-2 Haplotype
ES Cell lineEGR-05 [129S2]
Background strainJcl:B6D2F1
Appearance
Strain developmentDeveloped by Naokazu Inoue, Research Institute for Microbial Diseases, Osaka University in 2012. A frt flanked neo cassette was removed by crossing with CAG-FLPe mice (B6). C57BL/6, DBA/2 and 129 mixed background.
Strain descriptionIgsf8 floxed mice. Exons 3 to 5 were flanked by loxP sites.
Colony maintenanceHeterozygote x Wild-type [or Crossing to Jcl:B6D2F1]
References
Tetraspanin-interacting protein IGSF8 is dispensable for mouse fertility.
Inoue N, Nishikawa T, Ikawa M, Okabe M
Fertil. Steril., 98(2):465-470 (2012). 22609062

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site1Frt
Igsf8
MGI:2154090		immunoglobulin superfamily, member 81Igsf8<tm1.1Osb> targeted mutation 1.1, Research Institute for Microbial Diseases, Osaka University
loxP phage P1 loxP1loxP
loxP phage P1 loxP1loxP

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • reproductive system phenotype (MP:0005389)
    • Detailed phenotype data

    Ordering Information

    Donor DNAP1 phage loxP sites, yeast FRT (flipase recombination target) site, mouse Igsf8 genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Fertil. Steril., 98(2):465-470 (2012).
    DepositorNaokazu Inoue (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Aug 2015

    BRC mice in Publications

    Jimbo K, Nakajima-Takagi Y, Ito T, Koide S, Nannya Y, Iwama A, Tojo A, Konuma T.
    Immunoglobulin superfamily member 8 maintains myeloid leukemia stem cells through inhibition of β-catenin degradation.
    Leukemia 36(6) 1550-1562(2022) 35418614