Strain Data Sheet

RBRC05638

Strain Information

Image
BRC No.RBRC05638
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Nov<tm1Hko>
Former Common nameCCN3 KO, B6-Nov KO, B6;129-Nov<tm1Hko>, B6;129-Nov<tm1Hko>/HkoRbrc
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Haruhiko Koseki, RIKEN Center for Allergy and Immunology. R1 ES cells derived from (129X1/SvJ x 129S1/Sv)F1-Kitl<+> were used for the generation of knockout mice. The mutant mice were crossed to C57BL/6J.
Strain descriptionNov (CCN3) gene knockout mice. Exons 1, 2 and 3 of Nov gene were replaced with a neomycin selection cassette. Homozygous mutant mice show no obvious abnormality. Homozygous mutant mice exhibit enhanced neointimal hyperplasia in vascular smooth muscles induced by endothelial injury.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
References
CCN3 inhibits neointimal hyperplasia through modulation of smooth muscle cell growth and migration.
Shimoyama T, Hiraoka S, Takemoto M, Koshizaka M, Tokuyama H, Tokuyama T, Watanabe A, Fujimoto M, Kawamura H, Sato S, Tsurutani Y, Saito Y, Perbal B, Koseki H, Yokote K
Arterioscler. Thromb. Vasc. Biol., 30, 675-682 (2010). 20139355

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ccn3
MGI:109185		cellular communication network factor 315Ccn3<tm1Hko>
MGI:5004902		targeted mutation 1, Haruhiko Koseki
neo neomycin resistance gene (E. coli)15 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal vascular smooth muscle physiology(MP:0005595)

  • abnormal vascular wound healing(MP:0004883)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E.coli Neomycin resistance gene, mouse Nov genomic DNA
    Research application
    Specific Term and ConditionsThe RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorHaruhiko Koseki (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data