Strain Data Sheet
RBRC05641
Strain Information | |
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| Image | |
| BRC No. | RBRC05641 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.129P2-Pla2g4a<tm1Tksh> |
| Former Common name | B6-Pla2g4a KO, cPLA2a KO |
| H-2 Haplotype | |
| ES Cell line | E14.1 [129P2/OlaHsd] |
| Background strain | C57BL/6JJcl |
| Appearance | |
| Strain development | Developed by Naonori Uozumi and Takao Shimizu, Faculty of Medicine, University of Tokyo in 1997. E14.1 ES cells derived from 129P2/PlaHsd were used. The mutant mice were backcrossed to C57BL/6JJcl. |
| Strain description | Pla2g4a gene knockout mice. A part of the exon encoding the lipase consensus motif and serine 288 of Pla2g4a was replaced with a PGK-neo cassette. Homozygous mutant mice show reduced allergic and autoimmune reactions and females of homozygous are infertile (showing abnormal parturition and decreased litter size). |
| Colony maintenance | Heterozygote x Wild-type [C57BL/6JJcl] Homozygous mutant females are infertile. |
| References | Reduced fertility and postischaemic brain injury in mice deficient in cytosolic phospholipase A2. Bonventre J V, Huang Z, Taheri M R, O'Leary E, Li E, Moskowitz M A, Sapirstein A Nature, 390, 622-625 (1997). 9403693Role of cytosolic phospholipase A2 in allergic response and parturition. Uozumi N, Kume K, Nagase T, Nakatani N, Ishii S, Tashiro F, Komagata Y, Maki K, Ikuta K, Ouchi Y, Miyazaki J, Shimizu T Nature, 390, 618-622 (1997). 9403692 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Pla2g4a MGI:1195256 | phospholipase A2, group IVA (cytosolic, calcium-dependent) | 1 | Pla2g4a<tm1Tksh> MGI:2385582 | targeted mutation 1, Takao Shimizu | |||
| neo | neomycin resistance gene (E. coli) | 1 | mouse phosphoglycerate kinase promoter (PGK promoter) | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | more 9 phenotypes |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Phosphoglycerate kinase polyA, mouse Pla2g4a genomic DNA |
| Research application | Immunology and Inflammation Research Mouse Models for Human Disease |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 390, 618-622 (1997). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
| Depositor | Takao Shimizu (The University of Tokyo) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |