Strain Data Sheet

RBRC05642

Strain Information

Image
BRC No.RBRC05642
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Hspa5<tm1Aoe>
Former Common nameMutant BiP KI, B6-Hspa5 KI/HAtag
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Tomohiko Aoe, Chiba University Graduate School of Medicine in 2007. R1 ES cells were used. A floxed neo cassette was removed by crossing with CAG-Cre (B6) mice. The mutant mice were crossed to C57BL/6J.
Strain descriptionBiP (Hspa5) gene knock-in mice. Knock-in mice expressing a HA-tag mutant BiP gene lacking the KDEL retrieval sequence. Homozygous mutant mice exhibit impaired surfactant secretion and die within a few hours after birth.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
Aberrant quality control in the endoplasmic reticulum impairs the biosynthesis of pulmonary surfactant in mice expressing mutant BiP.
Mimura N, Hamada H, Kashio M, Jin H, Toyama Y, Kimura K, Iida M, Goto S, Saisho H, Toshimori K, Koseki H, Aoe T
Cell Death Differ., 14, 1475-1485 (2007). 17464327

Altered quality control in the endoplasmic reticulum causes cortical dysplasia in knock-in mice expressing a mutant BiP.
Mimura N, Yuasa S, Soma M, Jin H, Kimura K, Goto S, Koseki H, Aoe T
Mol. Cell. Biol., 28, 293-301 (2008). 17954555

Dysfunction of the ER chaperone BiP accelerates the renal tubular injury.
Kimura K, Jin H, Ogawa M, Aoe T
Biochem. Biophys. Res. Commun., 366, 1048-1053 (2008). 18158912

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
HA human influenza virus HA (hemagglutinin) tag sequence2HA
Hspa5
MGI:95835		heat shock protein 52Hspa5<tm1Aoe>
MGI:3773635		targeted mutation 1, Tomohiko Aoe
loxP phage P1 loxP2loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal alveolar lamellar body morphology(MP:0010813)

  • abnormal pulmonary alveolus epithelial cell morphology(MP:0002273)

  • abnormal renal tubule morphology(MP:0002703)

  • abnormal surfactant secretion(MP:0004780)

  • abnormal type II pneumocyte morphology(MP:0002275)
    • more 11 phenotypes
  • atelectasis(MP:0001177)

  • cyanosis(MP:0001575)

  • decreased fetal size(MP:0004200)

  • dilated renal tubule(MP:0002705)

  • increased susceptibility to injury(MP:0005165)

  • neonatal lethality, complete penetrance(MP:0011087)

  • pallor(MP:0003717)

  • pulmonary alveolar hemorrhage(MP:0010909)

  • renal interstitial fibrosis(MP:0003215)

  • renal tubule atrophy(MP:0011346)

  • respiratory distress(MP:0001954)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMouse BiP gene, Human influenza hemagglutinin (HA) のHA tag 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3
    Research applicationCre/loxP system
    Developmental Biology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. Cell Death Differ., 14, 1475-1485 (2007). Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must contact the DEPOSITOR/DEVELOPER and notify him/her the research content. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from use of the BIOLOGICAL RESOURCE.
    DepositorTomohiko Aoe (Chiba University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data