Strain Data Sheet

RBRC05646

Strain Information

Image
BRC No.RBRC05646
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Plch2<tm1Kfu>/KfuRbrc
Former Common nameB6-Plch2 KO, PLCeta(Acc.No.CDB0463K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology and Fukami Kiyoko, Tokyo University of Pharmacy and Life Sciences.
Strain descriptionPLCeta2 (Plch2) gene knockout mice. The first to third exons of PLCeta2 gene were replaced with a loxP/LacZ/frt-flanked neo/loxP/pA cassette. Homozygous mutant mice show no obvious abnormality.
Colony maintenanceHeterozygote x Wild-type [C57BL/6N]
References
Phospholipase C-eta2 is highly expressed in the habenula and retina.
Kanemaru K, Nakahara M, Nakamura Y, Hashiguchi Y, Kouchi Z, Yamaguchi H, Oshima N, Kiyonari H, Fukami K
Gene Expr. Patterns, 10, 119-126 (2010). 20060933

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site4Frt
Frt yeast FRT (flippase recombination target) site4Frt
Plch2
MGI:2443078		phospholipase C, eta 24Plch2<tm1Kfu >
MGI:4440833		targeted mutation 1, Kiyoko Fukami
lacZ beta-galactosidase (E. coli)4
lox71 mutant lox sequence4lox71
loxP phage P1 loxP4loxP
neo neomycin resistance gene (E. coli)4 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 lox71 site, E. coli lacZ, rabbit beta globin genomic DNA, yeast FRT (flippase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neomycin resistance gene, Phage P1 loxP site, mouse Plch2 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from the Dr.Kiyoko Fukami (E-mail: kfukami@toyaku.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0463K and Web. Site (https://large.riken.jp/distribution/mutant-list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. Gene Expr. Patterns, 10, 119-126 (2010). (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
    DepositorRIKEN CDB (RIKEN CDB)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data