Strain Data Sheet

RBRC05649

Strain Information

Image
BRC No.RBRC05649
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S4-Plin5<tm1Tosu>/Rbrc
Former Common nameB6-Plin5 KO, B6.129S4-Plin5<tm1>
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Tomohiro Yamaguchi and Takashi Osumi, Graduate School of Life Science, University of Hyogo in 2007. J1 ES cells derived from 129S4/SvJae were used. The mutant mice were backcrossed to C57BL/6JJcl.
Strain descriptionPlin5 perilipin 5 gene knockout mice. Exons 2 to 4 were replaced with a floxed PGK-neo cassette. In homozygous mutant mice, heart lipid droplets were absent and the activity of fatty acid oxidation was increased.
Colony maintenance
References
MLDP, a novel PAT family protein localized to lipid droplets and enriched in the heart, is regulated by peroxisome proliferator-activated receptor alpha.
Yamaguchi T, Matsushita S, Motojima K, Hirose F, Osumi T
J. Biol. Chem., 281, 14232-14240 (2006). 16571721

Perilipin 5, a lipid droplet-binding protein, protects heart from oxidative burden by sequestering fatty acid from excessive oxidation.
Kuramoto K, Okamura T, Yamaguchi T, Nakamura T Y, Wakabayashi S, Morinaga H, Nomura M, Yanase T, Otsu K, Usuda N, Matsumura S, Inoue K, Fushiki T, Kojima Y, Hashimoto T, Sakai F, Hirose F, Osumi T
J. Biol. Chem., 287, 23852-23863 (2012). 22532565

Deficiency of a lipid droplet protein, perilipin 5, suppresses myocardial lipid accumulation, thereby preventing type 1 diabetes-induced heart malfunction.
Kuramoto K, Sakai F, Yoshinori N, Nakamura T Y, Wakabayashi S, Kojidani T, Haraguchi T, Hirose F, Osumi T
Mol. Cell. Biol., (2014). 24820416

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GH Growth hormone polyA (Bovine)17GH
Plin5
MGI:1914218		perilipin 517Plin5<tm1Tosu>
MGI:5440666		targeted mutation 1, Takashi Osumi
loxP phage P1 loxP17loxP
loxP phage P1 loxP17loxP
neo neomycin resistance gene (E. coli)17 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal fatty acid oxidation(MP:0010953)

  • abnormal heart ventricle morphology(MP:0005294)

  • abnormal lipid level(MP:0001547)

  • decreased cardiac muscle contractility(MP:0005140)

  • decreased fatty acids level(MP:0005282)
    • more 10 phenotypes
  • decreased liver free fatty acids level(MP:0010360)

  • decreased liver triglyceride level(MP:0009356)

  • decreased triglyceride level(MP:0005318)

  • hyperactivity(MP:0001399)

  • increased circulating free fatty acids level(MP:0001554)

  • increased fatty acid oxidation(MP:0014171)

  • increased liver free fatty acids level(MP:0010359)

  • increased liver triglyceride level(MP:0009355)

  • increased oxygen consumption(MP:0005289)

  • oxidative stress(MP:0003674)
    • Detailed phenotype data

    Ordering Information

    Donor DNABacteriophage P1 loxP sites, E. coli Neomycin resistance gene, Bovine Growth hormone gene polyadenylation site, Mouse Phosphoglycerate kinase (PGK) gene promoter, mouse perilipin5 genomic DNA
    Research applicationCardiovascular Research
    Cre/loxP system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 287, 23852-23863 (2012). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorTakashi Osumi (University of Hyogo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data