Strain Data Sheet

RBRC05650

Strain Information

Image
BRC No.RBRC05650
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S-Zrsr1<tm1Mok>
Former Common nameUNG, B6;129S-Zrsr1<tm1Mok>, u2af1-rs1 KO
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strain
Appearance
Strain developmentDeveloped by Motoya Katsuki, National Institutes of Natural Sciences (Institute of Medical Science, University of Tokyo).
Strain descriptionu2af1-rs1 (Zrsr1) gene knockout mice. The entire coding region and about 3/4 of the CpG islands including region II were replaced with a PGK-gpt-neo cassette.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
The oocyte-specific methylated region of the U2afbp-rs/U2af1-rs1 gene is dispensable for its imprinted methylation.
Sunahara S, Nakamura K, Nakao K, Gondo Y, Nagata Y, Katsuki M
Biochem. Biophys. Res. Commun., 268, 590-595 (2000). 10679248

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Zrsr2-ps1
MGI:98885		zinc finger (CCCH type), RNA binding motif and serine/arginine rich 2, pseudogene 111Zrsr2-ps1<tm1Mok>
MGI:2388701		targeted mutation 1, Motoya Katsuki
neo neomycin resistance gene (E. coli)11 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli xanthine phosphoribosyltransferase (Ecogpt), E. coli neomycin resistance gene, mouse u2af1-rs1 genomic DNA
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. Biophys. Res. Commun., 268, 590-595 (2000).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Motoya Katsuki is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorToshikuni Sasaoka (Niigata University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data