Strain Data Sheet

RBRC05655

Strain Information

Image
BRC No.RBRC05655
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Cbl<tm1(CBL*Q367P)Homy>
Former Common nameCbl Q367P cKI, B6-Cbl/CBL*Q367P KI
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University in 2012. E14 ES cells (129P2/OlaHsd) were used. The mutant mice were crossed to C57BL/6 mice. Neomycin selection cassette was removed by crossing with FLPe mice.
Strain descriptionExon 5 to 9 of Cbl (Casitas B-lineage lymphoma) gene was replaced with a loxP flanked human CBL, and a human CBL having Q367P variant was inserted the downstream.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrlCrlj]
References
Acquired expression of Cbl(Q367P) in mice induces dysplastic myelopoiesis mimicking chronic myelomonocytic leukemia.
Nakata Y, Ueda T, Nagamachi A, Yamasaki N, Ikeda K I, Sera Y, Takubo K, Kanai A, Oda H, Sanada M, Ogawa S, Tsuji K, Ebihara Y, Wolff L, Honda Z I, Suda T, Inaba T, Honda H
Blood, 129, 2148-2160 (2017). 28209720

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
CBL Casitas B-lineage lymphoma(human)9CBL
Cbl
MGI:88279		Casitas B-lineage lymphoma9Cbl Casitas B-lineage lymphoma
  • CBL-related disorder(MedGEN)

  • juvenile myelomonocytic leukemia(DisGeNET, MedGEN)
    • Frt yeast FRT (flippase recombination target) site9Frt
    loxP phage P1 loxP9loxP
    loxP phage P1 loxP9loxP

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal cell cycle(MP:0003077)

  • abnormal circulating chemokine level(MP:0010209)

  • abnormal hematopoietic system physiology(MP:0001545)

  • abnormal leukocyte cell number(MP:0000217)

  • abnormal leukocyte morphology(MP:0008246)
    • more 10 phenotypes
  • enlarged spleen(MP:0000691)

  • increased acute myeloid leukemia incidence(MP:0020838)

  • increased chronic myelocytic leukemia incidence(MP:0005481)

  • increased common myeloid progenitor cell number(MP:0013891)

  • increased granulocyte monocyte progenitor cell number(MP:0013696)

  • increased hematopoietic stem cell number(MP:0004809)

  • increased leukemia incidence(MP:0002026)

  • increased megakaryocyte progenitor cell number(MP:0020463)

  • increased myeloid cell number in bone marrow(MP:0013415)

  • increased myeloid cell number(MP:0013663)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, human CBL cDNA, human CBL<Q367P> cDNA, yeast FRT (flippase recombination target) site, mouse Cbl genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorHiroaki Honda (Tokyo Women's Medical University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data