Strain Data Sheet

RBRC05658

Strain Information

Image
BRC No.RBRC05658
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Siglech<tm1.1Ksat>
Former Common nameSiglech<dtr>, B6-Siglech/DT-GFP KI
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Katsuaki Sato, RIKEN Research Center for Allergy and Immunology in 2009. Bruce 4 ES cells derived from B6.Cg-Thy1<a> were used. The mutant mice were backcross to C57BL/6J.
Strain descriptionIRES-DTR-EGFP cassette were inserted in to 3’ untranslated region of Siglech, Siglech sialic acid binding Ig-like lectin H gene. Plasmacytoid dendritic cells (pDCs) from homozygous mutant mice are defect in the transcriptional and cell surface expression of Siglech. In mice with diphtheria toxin (DT) induced specific ablation of pDCs.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6J(Crlj)]
References
Plasmacytoid dendritic cells are crucial for the initiation of inflammation and T cell immunity in vivo.
Takagi H, Fukaya T, Eizumi K, Sato Y, Sato K, Shibazaki A, Otsuka H, Hijikata A, Watanabe T, Ohara O, Kaisho T, Malissen B, Sato K
Immunity, 35, 958-971 (2011). 22177923

Critical role of plasmacytoid dendritic cells in induction of oral tolerance.
Uto T, Takagi H, Fukaya T, Nasu J, Fukui T, Miyanaga N, Arimura K, Nakamura T, Choijookhuu N, Hishikawa Y, Sato K
J. Allergy Clin. Immunol., (2018). 29477579

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)7EGFP
HBEGF heparin-binding EGF-like growth factor (human)7HBEGF
IRES2 internal ribosomal entry site (EMCV)7IRES2
Siglech
MGI:2443256		sialic acid binding Ig-like lectin H7Siglech<tm1.1Ksat>
MGI:5308226		targeted mutation 1.1, Katsuaki Sato
loxP phage P1 loxP7loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal circulating cytokine level(MP:0010210)

  • abnormal dendritic cell antigen presentation(MP:0002455)

  • abnormal plasmacytoid dendritic cell physiology(MP:0008769)

  • abnormal regulatory T cell morphology(MP:0004941)

  • decreased T-helper 17 cell number(MP:0010220)
    • more 8 phenotypes
  • decreased circulating interferon-alpha level(MP:0008574)

  • decreased circulating interleukin-12b level(MP:0008648)

  • decreased plasmacytoid dendritic cell number(MP:0008124)

  • decreased susceptibility to bacterial infection induced morbidity/mortality(MP:0009789)

  • decreased susceptibility to infection induced morbidity/mortality(MP:0009786)

  • increased T-helper 17 cell number(MP:0010219)

  • increased circulating interferon-alpha level(MP:0008573)

  • increased circulating interleukin-12b level(MP:0008647)
    • Detailed phenotype data

    Ordering Information

    Donor DNAEncephalomyocarditis virus (EMCV) internal ribosomal entry site 2 (ires2), human diphtheria toxin receptor (DTR) corresponds to the heparin binding EGF-like growth factor, Aequorea Victoria EGFP, Phage P1 loxP site, mouse Siglech genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Immunology and Inflammation Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Immunity, 35, 958-971 (2011). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR (Dr. Katsuaki Sato). The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a “MTA for Siglech-IRES2DTREGFP Knock-In Micefor Academic Research Purposes" specified by the DEPOSITOR and Inserm.
    DepositorKatsuaki Sato (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. MTA for Siglech-IRES2DTREGFP Knock-In Mice for Academic Research Purposes (English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Depositor's present address:Division of Immunology, Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki.
    Genotyping protocol -PCR-

    BRC mice in Publications

    Li C, Konishi H, Nishiwaki K, Sato K, Miyata T, Kiyama H.
    A mouse model of microglia-specific ablation in the embryonic central nervous system.
    Neurosci Res 173 54-61(2021) 34157360
    Konishi H, Okamoto T, Hara Y, Komine O, Tamada H, Maeda M, Osako F, Kobayashi M, Nishiyama A, Kataoka Y, Takai T, Udagawa N, Jung S, Ozato K, Tamura T, Tsuda M, Yamanaka K, Ogi T, Sato K, Kiyama H.
    Astrocytic phagocytosis is a compensatory mechanism for microglial dysfunction.
    EMBO J 39(22) e104464(2020) 32959911
    Koda Y, Nakamoto N, Chu PS, Ugamura A, Mikami Y, Teratani T, Tsujikawa H, Shiba S, Taniki N, Sujino T, Miyamoto K, Suzuki T, Yamaguchi A, Morikawa R, Sato K, Sakamoto M, Yoshimoto T, Kanai T.
    Plasmacytoid dendritic cells protect against immune-mediated acute liver injury via IL-35.
    J Clin Invest 129(8) 3201-3213(2019) 31264967