Strain Data Sheet
RBRC05660
Strain Information | |
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| Image | |
| BRC No. | RBRC05660 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | B6.B6D2-Tg(CAG-CAT,spH)5Yyan |
| Former Common name | CATV-5, B6-CAG-CAT-SpH Tg#5, B6.D2-Tg (CAG-cat, -spH) 5Yyan |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Developed by Yuchio Yanagawa, Gunma University Graduate School of Medicine and Hiromu Yawo, Tohoku University Graduate School of Life Sciences in 2002-2003. Transgene was injected into the introduced into the pronuclei of B6D2F1 fertilized eggs. The Tg mice were crossed to C57BL/6J. |
| Strain description | Vesicular exocytosis at presynaptic terminals is fundamental function for neurotransmission and brain function. The lumen of a secretory vesicle is acidic (pH 5.6), whereas it becomes neutral (pH 7.4) instantaneously upon exocytosis. The intravesicular change of pH can be detected by a fluorescence change of a pH-sensitive derivative of green fluorescent protein (pHluorin). SynaptopHluorin (SpH) is a fusion protein of pHluorin and syntaxin (VAMP2) for targeting to secretory vesicles. CAG-CAT-SpH transgenic mouse expresses SpH under the control of CAG promoter by cre-mediated recombination. This strain is useful for studies of presynaptic terminals in various regions of the brain. Tg mice mice show no obvious abnormality. |
| Colony maintenance | Carrier x Noncarrier [C57BL/6JJmsSlc] |
| References | Transgenic mouse lines expressing synaptopHluorin in hippocampus and cerebellar cortex. Araki R, Sakagami H, Yanagawa Y, Hikima T, Ishizuka T, Yawo H Genesis 42, 53-60 (2005). 15880564 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Vamp2 | vesicle-associated membrane protein 2 | UN | Vamp2 | ||||
| cat | E. coli chloramphenicol acetyltransferase (CAT) | UN | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) | ||||
| loxP | phage P1 loxP | UN | loxP | ||||
| loxP | phage P1 loxP | UN | loxP | ||||
| pHluorin | pH-sensitive Green Fluorescent Protein (Aequorea victoria) | UN | pHluorin | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), P1 phage loxP sites, E. coli Chloramphenicol acetyltransferase (CAT), SV40 Small T antigen polyA, mouse vesicular membrane protein Vamp2 cDNA, Jellyfish pH-sensitive mutants GFP cDNA (pHluorin cDNA(Miesenbck, G., De Angelis, D.A., Rothman, J. E. Nature, 394, 192-195, 1998).) |
| Research application | Cre/loxP system Fluorescent Proteins/lacZ System Neurobiology Research |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis 42, 53-60 (2005). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Any profit organizations cannot use the BIOLOGICAL RESOURCE without contracting license agreements with TOHOKU University. |
| Depositor | Hiromu Yawo (Tohoku University) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |