Strain Data Sheet

RBRC05661

Strain Information

Image
BRC No.RBRC05661
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Pcp4<tm1.1>
Former Common namePcp4-flox, B6-Pcp4 floxed
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Toshitada Takemori, RIKEN Center for Allergy and Immunology in 2009. Bruce 4 ES cells derived from B6.Cg-Thy1.1 were used. A frt flanked neo cassette was removed by crossing with CAG-FLPe mice#23 (B6).
Strain descriptionPcp4 floxed mice. Econ 3 was flanked by loxP sites.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site16Frt
Pcp4
MGI:97509		Purkinje cell protein 416Pcp4 <tm1.1Mtto> targeted mutation 1.1, Toshitada Takemori
loxP phage P1 loxP16loxP
loxP phage P1 loxP16loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Pcp4 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Immunology and Inflammation Research
    Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Akiko Ishige is requested.
    DepositorToshitada Takemori (RIKEN)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data