Strain Data Sheet

RBRC05668

Strain Information

Image
BRC No.RBRC05668
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameB10.Cg-H2<k> Tg(H2-Ea-Cd74/MCC*T102E)29Nnak
Former Common nameIi-102E, B10.BR-Ii-102E Tg#29
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Naoko Nakano, Institute of Genetics and Molecular and Cellular Biology in 1996. Transgene was injected into the pronuclei of (C57BL/6 x SJL) F2 fertilized eggs. The mice were backcrossed to B10.BR over 10 times.
Strain descriptionIi-102E transgenic mice expressing a fusion protein of Cd74 (MHC II invariant chain) and moth cytochrome c (MCC) 88-103 peptides sequence containing a single amino acid substitution at position 102 (threonine to glutamine).
Colony maintenanceCarrier x Noncarrier [or Crossing to B10.BR/SgSnSlc]
References
Development of CD25(+) T cells secreting transforming growth factor-beta1 by altered peptide ligands expressed as self-antigens.
Yamashiro H, Hozumi N, Nakano N
Int. Immunol., 14, 857-865 (2002). 12147622

Role of antigen persistence and dose for CD4+ T-cell exhaustion and recovery.
Han S, Asoyan A, Rabenstein H, Nakano N, Obst R
Proc. Natl. Acad. Sci. USA, 107, 20453-20458 (2010). 21059929

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
H2
MGI:95894		histocompatibility-2, MHC17H2<k>
MGI:3579322		k variant
Cd74
MGI:96534		CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated)UNCd74 mouse MHC class II E alpha promoter
MCC Moth cytochrome CUNMCC
Tg(H2-Ea-Cd74/MCC*T102E)29Nnak
MGI:4439131		UNTg(H2-Ea-Cd74/MCC*T102E)29Nnak transgene insertion 29, Naoko Nakano

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal negative T cell selection(MP:0004918)

  • decreased CD8-positive, alpha-beta T cell number(MP:0008079)

  • decreased susceptibility to autoimmune diabetes(MP:0004804)

  • increased CD4-positive, alpha-beta T cell number(MP:0008074)

  • increased physiological sensitivity to xenobiotic(MP:0008873)
    • more 3 phenotypes
  • increased susceptibility to autoimmune diabetes(MP:0004803)

  • periinsulitis(MP:0005580)

  • small thymus(MP:0000706)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse MHC class II E alpha promoter, mouse MHC class II invariant chain (Cd74), moth Moth cytochrome C (88-103 peptides) containing 102E substitution, rabbit beta-globin polyA
    Research applicationImmunology and Inflammation Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Int. Immunol., 14, 857-865 (2002).
    DepositorNaoko Nakano (Tokyo University of Science)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data