Strain Data Sheet

RBRC05681

Strain Information

Image
BRC No.RBRC05681
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Penk<tm1(KOMP)Mbp>/G01
Former Common name
H-2 Haplotype
ES Cell lineJM8A3.N1 [C57BL/6N-A<tm1Brd>]
Background strainC57BL/6NTac
Appearance
Strain development
Strain description
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
En2 En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)4En2
Frt yeast FRT (flippase recombination target) site4Frt
Frt yeast FRT (flippase recombination target) site4Frt
Ires internal ribosomal entry site (EMCV)4
Penk
MGI:104629		preproenkephalin4Penk<tm1(KOMP)Mbp>
MGI:4454042		targeted mutation 1, Mouse Biology Program, UCDavis
lacZ beta-galactosidase (E. coli)4
loxP phage P1 loxP4loxP
loxP phage P1 loxP4loxP
neo neomycin resistance gene (E. coli)4 human beta-actin promoter
SV40 polyA signal4

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • absent testes(MP:0006415)

  • decreased heart weight(MP:0002834)

  • decreased lean body mass(MP:0003961)

  • short tibia(MP:0002764)
    • Detailed phenotype dataPhenopub Link

    Ordering Information

    Donor DNAyeast FRT (flipase recombination target) sites, En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, SV40 polyadenylation site, phage P1 loxP sites, hBactP (human beta actin promoter), E. coli neo, mouse Penk genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT should acknowledge the KOMP, RIKEN BRC and the International Mouse Phenotyping Consortium as the original creator of the targeted mouse ES cells and the provider of the knockout mice, respectively.
    DepositorRIKEN BRC (RIKEN BRC)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data