Strain Data Sheet

RBRC05690

Strain Information

Image
BRC No.RBRC05690
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Sst<tm1a(KOMP)Wtsi>/F04
Former Common name
H-2 Haplotype
ES Cell lineJM8A3.N1 [C57BL/6N-A<tm1Brd>]
Background strainC57BL/6NTac
Appearance
Strain development
Strain description
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
En2 En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)16En2
Frt yeast FRT (flippase recombination target) site16Frt
Frt yeast FRT (flippase recombination target) site16Frt
Ires internal ribosomal entry site (EMCV)16
Sst
MGI:98326		somatostatin16Sst<tm1a(KOMP)Wtsi>
MGI:5286229		targeted mutation 1a, Wellcome Trust Sanger Institute
lacZ beta-galactosidase (E. coli)16
loxP phage P1 loxP16loxP
loxP phage P1 loxP16loxP
neo neomycin resistance gene (E. coli)16
SV40 polyA signal16

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAyeast FRT (flipase recombination target) sites, En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, SV40 polyadenylation site, phage P1 loxP sites, hBactP (human beta actin promoter), E. coli neo, mouse Sst genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT should acknowledge RIKEN BRC and the International Mouse Phenotyping Consortium for creating and providing of the knockout mice.
    DepositorRIKEN BRC (RIKEN BRC)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data