Strain Data Sheet

RBRC05691

Strain Information

Image
BRC No.RBRC05691
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Fgf18<tm1Tri>
Former Common nameFgf18 floxed, B6-Fgf18 floxed
H-2 Haplotype
ES Cell lineC6 [129/SvEvTac]
Background strain
Appearance
Strain developmentDeveloped by Toru Imamura, National Institute of Advanced Industrial Science and Technology in 2009. A FRT-flanked PGK-neo cassette with a 5’loxP site was inserted upstream of exon 3 and a loxP site was inserted downstream of exon 3. A PGK-neo cassette was removed by crossing with FLPe mice (B6-Tg(CAG-FLPe)36 RBRC01834).
Strain descriptionFgf18 gene floxed mice. Exon 3 of Fgf18 gene was flanked by loxP sites. Conditional Fgf18 deficient mice can be generated by crossing with tissue-specific Cre mice. Homozygous mice are viable and fertile. Sometimes curly hairs.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6]
References
Hair cycle resting phase is regulated by cyclic epithelial FGF18 signaling.
Kimura-Ueki M, Oda Y, Oki J, Komi-Kuramochi A, Honda E, Asada M, Suzuki M, Imamura T
J. Invest. Dermatol., 132, 1338-1345 (2012). 22297635

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Fgf18
MGI:1277980		fibroblast growth factor 1811Fgf18<tm1Tri>
MGI:5425911		targeted mutation 1, Toru Imamura
Frt yeast FRT (flippase recombination target) site11Frt
loxP phage P1 loxP11loxP
loxP phage P1 loxP11loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal coat appearance(MP:0001510)

  • abnormal hair cycle anagen phase(MP:0008858)

  • abnormal hair cycle telogen phase(MP:0008860)

  • abnormal hair cycle(MP:0000427)

  • integument phenotype(MP:0010771)
    • Detailed phenotype data

    Ordering Information

    Donor DNABacteriophage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Fgf18 genomic DNA
    Research applicationCre/loxP system
    Dermatology Research
    Developmental Biology Research
    FLP/frt system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Invest. Dermatol., 132, 1338-1345 (2012). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For use of the BIOLOGICAL RESOURCE, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorYoshihiro Ohmiya (National Institute of Advanced Industrial Science and Technology)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Tsuchiya Y, Seki T, Kobayashi K, Komazawa-Sakon S, Shichino S, Nishina T, Fukuhara K, Ikejima K, Nagai H, Igarashi Y, Ueha S, Oikawa A, Tsurusaki S, Yamazaki S, Nishiyama C, Mikami T, Yagita H, Okumura K, Kido T, Miyajima A, Matsushima K, Imasaka M, Araki K, Imamura T, Ohmuraya M, Tanaka M, Nakano H.
    Fibroblast growth factor 18 stimulates the proliferation of hepatic stellate cells, thereby inducing liver fibrosis.
    Nat Commun 14(1) 6304(2023) 37813881