Strain Data Sheet

RBRC05692

Strain Information

Image
BRC No.RBRC05692
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Trpc3<tm1b(EUCOMM)Wtsi>/D08
Former Common name
H-2 Haplotype
ES Cell lineJM8A3.N1 [C57BL/6N-A<tm1Brd>]
Background strainC57BL/6NTac
Appearance
Strain development
Strain description
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
En2 En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)3En2
Frt yeast FRT (flippase recombination target) site3Frt
IRES internal ribosomal entry site (EMCV)3
Trpc3
MGI:109526		transient receptor potential cation channel, subfamily C, member 33Trpc3<tm1b(EUCOMM)Wtsi> targeted mutation 1b, Wellcome Trust Sanger Institute
  • spinocerebellar ataxia type 41(MedGEN)
    • lacZ beta-galactosidase (E. coli)3
    loxP phage P1 loxP3loxP
    SV40 polyA signal3

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • preweaning lethality, complete penetrance(MP:0011100)
    • Detailed phenotype data

    Ordering Information

    Donor DNAyeast FRT (flipase recombination target) site, En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, SV40 polyadenylation site, phage P1 loxP site, mouse Trpc3 genomic DNA
    Research application
    Specific Term and ConditionsThe RECIPIENT should acknowledge the EUCOMM, RIKEN BRC and the International Mouse Phenotyping Consortium as the original creator of the targeted mouse ES cells and the provider of the knockout mice, respectively.
    DepositorRIKEN BRC (RIKEN BRC)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data