Strain Data Sheet

RBRC05701

Strain Information

Image
BRC No.RBRC05701
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameSTOCK Tg(FLEXCAG-VSFP-Butterfly)552Bsi
Former Common name
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Dr. Thomas Knoepfel, RIKEN Brain Science Institute.
Strain descriptionMice carry the gene for the voltage sensor, VSFP-Butterfly flanked by loxP sites. Expression will be activate by cre recombinase following crossing with a cre driver mouse.
Colony maintenanceCarrier x Carrier [or Crossing to C57BL/6JJcl]
References
Genetically encoded optical indicators for the analysis of neuronal circuits.
Knopfel T
Nat. Rev. Neurosci., 13, 687-700 (2012). 22931891

Imaging neural circuit dynamics with a voltage-sensitive fluorescent protein.
Akemann W, Mutoh H, Perron A, Park Y K, Iwamoto Y, Knopfel T
J. Neurophysiol., 108, 2323-2337 (2012). 22815406

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ci-VSP Ciona intestinalis voltage sensor-containing phosphataseUNCi-VSP
WPRE Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)UNWPRE
lox2272 phage P1 loxP variantUNlox2272
lox2272 phage P1 loxP variantUNlox2272
loxP phage P1 loxPUNloxP
loxP phage P1 loxPUNloxP
mCitrine Yellow Fluorescent Protein (Aequorea victoria)UNmCitrine
mKate Red Fluorescent Protein (Entacmaea quadricolor)UNmKate CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAAequorea victoria Green Fluorescent Protein and derivatives coding sequence, Ciona intestinalis Ci-VSP, Entacmaea quadricolor Far-red Fluorescent Protein (mKate) and derivatives coding sequence, Bovine growth hormone polyadenylation (poly-A) signal, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), phage P1 loxP, Woodchuck hepatitis virus post-transcriptional regulatory element (WPRE)
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorThomas Knöepfel (RIKEN)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form ( English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)
    Contact Information
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data