Strain Data Sheet

RBRC05702

Strain Information

Image
BRC No.RBRC05702
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(CAG-XBP1/luc)23Tiw
Former Common nameERAI-LUC, B6-ERAI/Luc Tg#23
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Takao Iwawaki, Advanced Science Institute, RIKEN in 2005. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs. The mice were backcrossed to C57BL/6J.
Strain descriptionERAI (ER stress-activated indicator) transgenic mice carry human XBP1 and luciferase fusion gene under control of a CA promoter (cytomegalovirus enhancer and chicken beta-actin promoter). Endoplasmic reticulum (ER) stress is caused by the accumulation of unfolded proteins in the ER lumen, and is associated with vascular and neurodegenerative diseases. The transcripts from ERAI constructs are spliced under ER stress, the spliced mRNA is translated into an XBP1-luc fusion protein, which can be detected by fluorescence of luciferase that mediated by luciferin substrate injection. This strain is useful to monitor physiological and pathological ER stress in vivo.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
References
Function of IRE1 alpha in the placenta is essential for placental development and embryonic viability.
Iwawaki T, Akai R, Yamanaka S, Kohno K
Proc. Natl. Acad. Sci. USA, 106, 16657-16662 (2009). 19805353

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
FLAG FLAG tag (synthetic)UNFLAG
XBP1 X-box binding protein 1UNXBP1 CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
luc luciferase (firefly)UNluc

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), FLAG tag, human XBP1 cDNA, firefly luciferase, SV40 intron and polyA signal
    Research applicationCell Biology Research
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 106, 16657-16662 (2009). The RECIPIENT shall use the BIOLOGICAL RESOURCE in collaboration with the DEPOSITOR (Dr. Takao Iwawaki).
    DepositorTakao Iwawaki (Gunma University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Jan 2013

    BRC mice in Publications

    No Data