Strain Data Sheet

RBRC05708

Strain Information

Image
BRC No.RBRC05708
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Mstn<tm1.1Hko>/HkoRbrc
Former Common nameGdf8 delta lox/+ (GWAS002), B6-Gdf8 KO, B6.Cg-Gdf8<tm1.1Hko>
H-2 Haplotype
ES Cell lineM1 [(C57BL/6 x 129)F1]
Background strain
Appearance
Strain developmentDeveloped by Dr. Haruhiko Koseki, RIKEN Center for Allergy and Immunology. M1 ES cells derived from (C57BL/6 x 129))F1-Kitl<+> were used for the generation of knockout mice. Exon 2 and neo cassette were removed by crossing with CAG-Cre mice (C57BL/6 background). The mutant mice were crossed to C57BL/6J.
Strain descriptionGdf8 (Mstn) gene knockout mice. Exon 2 was removed. Homozygous mice show no obvious abnormality.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Mstn
MGI:95691		myostatin1Mstn<tm1.1Hko> targeted mutation 1.1, Haruhiko Koseki
  • myostatin-related muscle hypertrophy(MedGEN)
    • loxP phage P1 loxP1loxP

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAP1 Phage loxP site, Mouse Gdf8 (Mstn) genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsThe RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorHaruhiko Koseki (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data