Strain Data Sheet

RBRC05711

Strain Information

Image
BRC No.RBRC05711
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameSTOCK Tg(tetO-OPN4,mCherry)M5
Former Common nameBitetO-OPN4/mCherry
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Tomomi Tsunematsu, Kenji F. Tanaka Akihiro Yamanaka and Amane Koizumi, National Institute for Physiological Sciences in 2012. The transgene was injected into the pronuclei of CBA/C57BL/6 fertilized eggs. Mixed genetic background.
Strain descriptionBitetO-OPN4/mCherry transgenic mice. These transgenic mice express human OPN4 and mCherry genes under the control of the bi-directional tet-responsive element.
Colony maintenanceCarrier x Carrier [or Crossing to C57BL/6JJcl]
References
Ectopic expression of melanopsin in orexin/hypocretin neurons enables control of wakefulness of mice in vivo by blue light.
Tsunematsu T, Tanaka K F, Yamanaka A, Koizumi A
Neurosci. Res., 75(1):23-28 (2012). 22868039

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
OPN4 opsin 4 (melanopsin)(human)UNOPN4 E. coli Tetracycline operator site, Cytomegalovirus CMV minimum promoter
mCherry Red Fluorescent Protein (Discosoma sp.)UNmCherry

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli Tetracycline operator site, Human Opn4 gene cDNA, Cytomegalovirus CMV minimum promoter, SV40 PolyA signal, Discosoma mCherry gene cDNA
    Research applicationFluorescent Proteins/lacZ System
    Neurobiology Research
    Tet system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neurosci. Res., 75(1):23-28 (2012).
    DepositorAmane Koizumi (National Institute for Physiological Sciences)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. UCSD MTA
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data