Strain Data Sheet

RBRC05713

Strain Information

Image
BRC No.RBRC05713
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S7-Asgr1<tm1Ishi>/Tats
Former Common nameAsgr1 KO
H-2 Haplotype
ES Cell lineJH1 [129S7/SvEvBrd]
Background strain
Appearance
Strain developmentDeveloped by Shun Ishibashi, Faculty of Medicine, University of Tokyo in 2001. JH1 ES cells derived from 129S7/SvEvBrd were used for the generation of knockout mice. The mice were backcrossed to C57BL/6J for 8 generations by Laboratory of Cancer Biology and Molecular Immunology, University of Tokyo.
Strain descriptionAsgr1 knockout mice. The transcription initiation codon and the sequences encoding the transmembrane domain of Asgr1 gene were replaced with a pol2neo cassette. Homozygous mice show an impaired hepatic clearance of asialoglycoproteins.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
Asialoglycoprotein receptor deficiency in mice lacking the major receptor subunit. Its obligate requirement for the stable expression of oligomeric receptor.
Tozawa R, Ishibashi S, Osuga J, Yamamoto K, Yagyu H, Ohashi K, Tamura Y, Yahagi N, Iizuka Y, Okazaki H, Harada K, Gotoda T, Shimano H, Kimura S, Nagai R, Yamada N
J. Biol. Chem., 276, 12624-12628 (2001). 11278827

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Asgr1
MGI:88081		asialoglycoprotein receptor 111Asgr1<tm1Ishi>
MGI:2180369		targeted mutation 1, Shun Ishibashi

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal liver physiology(MP:0000609)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli Neomycin resistance gene, Mouse Asgr1 genomic DNA, bovine Growth hormone polyA, Mouse RNA polymerase II promoter
    Research applicationCell Biology Research
    Metabolism Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 276, 12624-12628 (2001).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorTatsuro Irimura (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data