Strain Data Sheet

RBRC05737

Strain Information

Image
BRC No.RBRC05737
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(CAG-tdKaede)15Utr
Former Common nameWK-m15
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Dr. Yoshihiro Miwa, Graduate School of Comprehensive Human Sciences, University of Tsukuba in 2007. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The Kaede cDNA was provided by Dr. Atsushi Miyawaki, RIKEN Brain Science Institute. C57BL/6 background (RBRC05737), albino C57BL/6 background (RBRC05738).
Strain descriptionThese transgenic mice expressing a Kaede, photoconvertible fluorescence protein under the control of the CAG promoter. The expression of green fluoresce of Kaede mice can be converted to red on irradiation with UV or violet light.
Colony maintenanceCarrier x Carrier [Homozygote x Homozygote]
References
Tyrosinase subcellular distribution and kinetic parameters in wild type and C-locus mutant C57BL/6J mice.
Townsend D, Witkop C J Jr, Mattson J
J. Exp. Zool., 216, 113-119 (1981). 6793688

Monitoring cellular movement in vivo with photoconvertible fluorescence protein "Kaede" transgenic mice.
Tomura M, Yoshida N, Tanaka J, Karasawa S, Miwa Y, Miyawaki A, Kanagawa O
Proc. Natl. Acad. Sci. USA, 105(31):10871-10876 (2008). 18663225

Health Report

Examination Date / Room / Rack2025/01/07Room:3-ARack:CSentinel mouse program
2024/10/07Room:3-ARack:CSentinel mouse program
2024/07/08Room:3-ARack:CSentinel mouse program
2024/04/08Room:3-ARack:CSentinel mouse program
2024/01/09Room:3-ARack:CSentinel mouse program
2023/12/21Room:3-ARack:CAdditional tests
2023/10/10Room:3-ARack:CSentinel mouse program
2023/07/10Room:3-ARack:CSentinel mouse program
2023/04/10Room:3-ARack:CSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
tdKaede Trachyphyllia geoffroy Kaede cDNAUNtdKaede CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
SV40 polyA siteUN

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Trachyphyllia geoffroy Kaede cDNA, SV40 Poly(A) site
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 105, 10871-10876 (2008). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research.
    In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Kaede, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested. Proc. Natl. Acad. Sci. USA, 99(20) 12651–12656 (2002).
    DepositorYoshihiro Miwa (University of Tsukuba)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (3 to 6 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. CAGGS MTA (English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Aug 2016
    Mouse of the Month Dec 2020

    BRC mice in Publications

    Lamorte S, Quevedo R, Jin R, Neufeld L, Liu ZQ, Ciudad MT, Lukhele S, Bruce J, Mishra S, Zhang X, Saeed ZK, Berman H, Philpott DJ, Girardin SE, Harding S, Munn DH, Mak TW, Karlsson MCI, Brooks DG, McGaha TL.
    Lymph node macrophages drive immune tolerance and resistance to cancer therapy by induction of the immune-regulatory cytokine IL-33.
    Cancer Cell (2025) 40054466
    Johansson A, Khalilnezhad A, Takizawa H, Mizuno H, Suda T, Umemoto T.
    Mobilization dynamics of bone marrow hematopoietic stem cells during hematopoietic regeneration.
    Exp Hematol 138 104281(2024) 39009278
    Nakamura YK, Janowitz C, Metea C, Asquith M, Karstens L, Rosenbaum JT, Lin P.
    Short chain fatty acids ameliorate immune-mediated uveitis partially by altering migration of lymphocytes from the intestine.
    Sci Rep 7(1) 11745(2017) 28924192