Strain Data Sheet

RBRC05738

Strain Information

Image
BRC No.RBRC05738
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-c/c Tg(CAG-tdKaede)15Utr
Former Common nameWK-m15(Tyrc-2J)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Dr. Yoshihiro Miwa, Graduate School of Comprehensive Human Sciences, University of Tsukuba in 2007. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The Kaede cDNA was provided by Dr. Atsushi Miyawaki, RIKEN Brain Science Institute. C57BL/6 background (RBRC05737), albino C57BL/6 background (RBRC05738).
Strain descriptionThese transgenic mice expressing a Kaede, photoconvertible fluorescence protein under the control of the CAG promoter. The expression of green fluoresce of Kaede mice can be converted to red on irradiation with UV or violet light.
Colony maintenanceCarrier x Carrier
References
Tyrosinase subcellular distribution and kinetic parameters in wild type and C-locus mutant C57BL/6J mice.
Townsend D, Witkop C J Jr, Mattson J
J. Exp. Zool., 216, 113-119 (1981). 6793688

Monitoring cellular movement in vivo with photoconvertible fluorescence protein "Kaede" transgenic mice.
Tomura M, Yoshida N, Tanaka J, Karasawa S, Miwa Y, Miyawaki A, Kanagawa O
Proc. Natl. Acad. Sci. USA, 105(31):10871-10876 (2008). 18663225

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Tyr
MGI:98880		tyrosinase7Tyr<c-2J>
MGI:1855985		albino 2 Jackson
  • oculocutaneous albinism type 1A(MedGEN)

  • oculocutaneous albinism type 1B(MedGEN)

  • oculocutaneous albinism(DisGeNET)
    • tdKaede Trachyphyllia geoffroy Kaede cDNAUNtdKaede CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
    SV40 polyA signalUN

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Trachyphyllia geoffroy Kaede cDNA, SV40 Poly(A) site
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 105, 10871-10876 (2008). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Kaede, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested. Proc. Natl. Acad. Sci. USA, 99(20) 12651–12656 (2002).
    DepositorYoshihiro Miwa (University of Tsukuba)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. CAGGS MTA (English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Aug 2016
    Mouse of the Month Dec 2020

    BRC mice in Publications

    Liang Z, Tang Z, Zhu C, Li F, Chen S, Han X, Zheng R, Hu X, Lin R, Pei Q, Yin C, Wang J, Tang C, Cao N, Zhao J, Wang R, Li X, Luo N, Wen Q, Yu J, Li J, Xia X, Zheng X, Wang X, Huang N, Zhong Z, Mo C, Chen P, Wang Y, Fan J, Guo Y, Zhong H, Liu J, Peng Z, Mao H, Shi GP, Bonventre JV, Chen W, Zhou Y.
    Intestinal CXCR6+ ILC3s migrate to the kidney and exacerbate renal fibrosis via IL-23 receptor signaling enhanced by PD-1 expression.
    Immunity (2024) 38815582