Strain Data Sheet

RBRC05753

Strain Information

Image
BRC No.RBRC05753
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S4-Map3k5<tm1Hijo>
Former Common nameASK1 KO, B6-Map3k5 KO
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Hidenori Ichijo, Graduate School, Tokyo Medical and Dental University in 2001. J1 ES cells were used. The mutant mice are backcrossed to C57BL/6J.
Strain descriptionMap3k5 gene knockout mice. The coding region of Exon 1 of Map3k5 gene was replaced with a GFP/PGK-neo cassette (GFP expression was not observed).
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl] Homozygous mutant mice are viable and fertile.
References
Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways.
Ichijo H, Nishida E, Irie K, ten Dijke P, Saitoh M, Moriguchi T, Takagi M, Matsumoto K, Miyazono K, Gotoh Y
Science., 275, 90-94 (1997). 8974401

ASK1 is required for sustained activations of JNK/p38 MAP kinases and apoptosis.
Tobiume K, Matsuzawa A, Takahashi T, Nishitoh H, Morita K, Takeda K, Minowa O, Miyazono K, Noda T, Ichijo H
EMBO Rep., 2, 222-228 (2001). 11266364

Health Report

Examination Date / Room / Rack2023/11/27Room:4-BRack:HSentinel mouse program
2023/08/30Room:4-BRack:HAdditional tests
2023/08/28Room:4-BRack:HSentinel mouse program
2023/05/29Room:4-BRack:HSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFP Green Fluorescent Protein (Aequorea victoria)10
GH Growth hormone polyA (Bovine)10GH
Map3k5
MGI:1346876		mitogen-activated protein kinase kinase kinase 510Map3k5<tm1Hijo>
MGI:3046838		targeted mutation 1, Hidenori Ichijo
neo neomycin resistance gene (E. coli)10 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal response to cardiac infarction(MP:0000343)

  • cardiovascular system phenotype(MP:0005385)

  • decreased physiological sensitivity to xenobiotic(MP:0008874)

  • decreased response of heart to induced stress(MP:0004486)

  • decreased sensitivity to induced cell death(MP:0008944)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMouse Phosphoglycerate kinase promoter (Pgk1 promoter), E. coli Neomycin resistance gene, mouse Pgk1 polyA signal, Jellyfish (Aequorea victoria) GFP cDNA, Bovine PolyA signal, Mouse Map3k5 genomic DNA
    Research applicationFluorescent Proteins/lacZ System
    Immunology and Inflammation Research
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
    DepositorHidenori Ichijo (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Aug 2014

    BRC mice in Publications

    No Data