Strain Data Sheet

RBRC05754

Strain Information

Image
BRC No.RBRC05754
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Map3k6<tm1Hijo>
Former Common nameASK2 KO, B6-Map3k6 KO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Hidenori Ichijo, Graduate School of Pharmaceutical Sciences, The University of Tokyo in 2009. E14 ES cells were used. The mutant mice are backcrossed to C57BL/6J.
Strain descriptionMap3k6 gene knockout mice. The exons 1 to 5 were replaced with a NLS-lacZ cassette and a pgk-neo cassette.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl] Homozygous mutant mice are viable and fertile.
References
Apoptosis signal-regulating kinase (ASK) 2 functions as a mitogen-activated protein kinase kinase kinase in a heteromeric complex with ASK1.
Takeda K, Shimozono R, Noguchi T, Umeda T, Morimoto Y, Naguro I, Tobiume K, Saitoh M, Matsuzawa A, Ichijo H
J. Biol. Chem., 282, 7522-75231 (2007). 17210579

ASK1 and ASK2 differentially regulate the counteracting roles of apoptosis and inflammation in tumorigenesis.
Iriyama T, Takeda K, Nakamura H, Morimoto Y, Kuroiwa T, Mizukami J, Umeda T, Noguchi T, Naguro I, Nishitoh H, Saegusa K, Tobiume K, Homma T, Shimada Y, Tsuda H, Aiko S, Imoto I, Inazawa J, Chida K, Kamei Y, Kozuma S, Taketani Y, Matsuzawa A, Ichijo H
EMBO J., 28, 843-853 (2009). 19214184

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Map3k6
MGI:1855691		mitogen-activated protein kinase kinase kinase 64Map3k6<tm1Hijo>
MGI:3843985		targeted mutation 1, Hidenori Ichijo
lacZ beta-galactosidase (E. coli)4
neo neomycin resistance gene (E. coli)4 mouse phosphoglycerate kinase promoter (PGK promoter)
nls SV40 large T antigen nuclear localization signal (NLS)4nls

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • decreased keratinocyte apoptosis(MP:0009581)

  • decreased physiological sensitivity to xenobiotic(MP:0008874)

  • increased incidence of tumors by chemical induction(MP:0004499)

  • integument phenotype(MP:0010771)

  • neoplasm(MP:0002006)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli Neomycin resistance gene, Mouse Phosphoglycerate kinase promoter (PGK promoter), Mouse Map3k6 genomic DNA, E. coli beta-galactosidase, SV40 nuclear localization signal sequence, Bovine PolyA signal
    Research applicationFluorescent Proteins/lacZ System
    Immunology and Inflammation Research
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
    DepositorHidenori Ichijo (The University of Tokyo)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data